A BAC contig map of the Ly49 gene cluster in 129 mice reveals extensive differences in gene content relative to C57BL/6 mice

Andrew P. Makrigiannis; Amanda T. Pau; Pamela L. Schwartzberg; Daniel W. McVicar; Thomas W. Beck; Stephen K. Anderson

doi:10.1006/geno.2002.6724

A BAC contig map of the Ly49 gene cluster in 129 mice reveals extensive differences in gene content relative to C57BL/6 mice

Andrew P. Makrigiannis, Amanda T. Pau, Pamela L. Schwartzberg, Daniel W. McVicar, Thomas W. Beck, Stephen K. Anderson

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

The murine Ly49 gene family is functionally analogous to the human killer cell Ig-like receptor (KIR) family of class I major histocompatibility complex (MHC) receptors. The number of KIR genes varies dramatically between individuals; however, the organization of the Ly49 genes has only been determined for the C57BL/6 (B6) mouse. The organization of the 129 Ly49 loci was determined from a BAC contig map by PCR and Southern blot analysis. In addition to the 10 Ly49 genes known from previous studies of the 129/J strain, 8 new genes were localized to the 129 Ly49 cluster. A gene order of Ly49q₁, e, (v, q₂), e/c₂, l/r, s, t, e/c₁, r, u, u/i, i₁, g, p/d, (i₂, p), and o was determined. The 129 Ly49 gene cluster is predicted to span ∼ 600 kb. These results indicate that Ly49 gene numbers can be significantly different between inbred mouse strains, analogous to the haplotype differences observed in the human KIR genes.

Original language	English
Pages (from-to)	437-444
Number of pages	8
Journal	Genomics
Volume	79
Issue number	3
DOIs	https://doi.org/10.1006/geno.2002.6724
Publication status	Published - 2002
Externally published	Yes

Bibliographical note

Funding Information:
This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. N01-C0-12400.

ASJC Scopus Subject Areas

Genetics

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10.1006/geno.2002.6724

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title = "A BAC contig map of the Ly49 gene cluster in 129 mice reveals extensive differences in gene content relative to C57BL/6 mice",

abstract = "The murine Ly49 gene family is functionally analogous to the human killer cell Ig-like receptor (KIR) family of class I major histocompatibility complex (MHC) receptors. The number of KIR genes varies dramatically between individuals; however, the organization of the Ly49 genes has only been determined for the C57BL/6 (B6) mouse. The organization of the 129 Ly49 loci was determined from a BAC contig map by PCR and Southern blot analysis. In addition to the 10 Ly49 genes known from previous studies of the 129/J strain, 8 new genes were localized to the 129 Ly49 cluster. A gene order of Ly49q1, e, (v, q2), e/c2, l/r, s, t, e/c1, r, u, u/i, i1, g, p/d, (i2, p), and o was determined. The 129 Ly49 gene cluster is predicted to span ∼ 600 kb. These results indicate that Ly49 gene numbers can be significantly different between inbred mouse strains, analogous to the haplotype differences observed in the human KIR genes.",

author = "Makrigiannis, {Andrew P.} and Pau, {Amanda T.} and Schwartzberg, {Pamela L.} and McVicar, {Daniel W.} and Beck, {Thomas W.} and Anderson, {Stephen K.}",

note = "Funding Information: This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. N01-C0-12400.",

year = "2002",

doi = "10.1006/geno.2002.6724",

language = "English",

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AU - Pau, Amanda T.

AU - Schwartzberg, Pamela L.

AU - McVicar, Daniel W.

AU - Beck, Thomas W.

AU - Anderson, Stephen K.

N1 - Funding Information: This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. N01-C0-12400.

PY - 2002

Y1 - 2002

N2 - The murine Ly49 gene family is functionally analogous to the human killer cell Ig-like receptor (KIR) family of class I major histocompatibility complex (MHC) receptors. The number of KIR genes varies dramatically between individuals; however, the organization of the Ly49 genes has only been determined for the C57BL/6 (B6) mouse. The organization of the 129 Ly49 loci was determined from a BAC contig map by PCR and Southern blot analysis. In addition to the 10 Ly49 genes known from previous studies of the 129/J strain, 8 new genes were localized to the 129 Ly49 cluster. A gene order of Ly49q1, e, (v, q2), e/c2, l/r, s, t, e/c1, r, u, u/i, i1, g, p/d, (i2, p), and o was determined. The 129 Ly49 gene cluster is predicted to span ∼ 600 kb. These results indicate that Ly49 gene numbers can be significantly different between inbred mouse strains, analogous to the haplotype differences observed in the human KIR genes.

AB - The murine Ly49 gene family is functionally analogous to the human killer cell Ig-like receptor (KIR) family of class I major histocompatibility complex (MHC) receptors. The number of KIR genes varies dramatically between individuals; however, the organization of the Ly49 genes has only been determined for the C57BL/6 (B6) mouse. The organization of the 129 Ly49 loci was determined from a BAC contig map by PCR and Southern blot analysis. In addition to the 10 Ly49 genes known from previous studies of the 129/J strain, 8 new genes were localized to the 129 Ly49 cluster. A gene order of Ly49q1, e, (v, q2), e/c2, l/r, s, t, e/c1, r, u, u/i, i1, g, p/d, (i2, p), and o was determined. The 129 Ly49 gene cluster is predicted to span ∼ 600 kb. These results indicate that Ly49 gene numbers can be significantly different between inbred mouse strains, analogous to the haplotype differences observed in the human KIR genes.

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A BAC contig map of the Ly49 gene cluster in 129 mice reveals extensive differences in gene content relative to C57BL/6 mice

Abstract

Bibliographical note

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