TY - JOUR
T1 - A comparison of fast-atom bombardment and electrospray as methods of ionization in the study of sulphated- and sulphonated-lipids by tandem mass spectrometry
AU - Griffiths, W. J.
AU - Brown, A.
AU - Reimendal, R.
AU - Yang, Y.
AU - Zhang, J.
AU - Sjövall, J.
PY - 1996
Y1 - 1996
N2 - In recent years electrospray has become the most popular ionization technique for the analysis of biomolecules. In the present study, we have compared the electrospray and continuous-flow fast-atom bombardment methods of sample introduction and ionization for the analysis of sulphated and sulphonated lipids by tandem moss spectroscopy. The tandem mass spectra obtained when using the two ionization techniques were similar; however, small differences were evident. These differences indicate that the precursor ions generated by fast-atom bombardment and electrospray have different internal energies. The two ionization techniques give similar sensitivities for the recording of collision-induced dissociation spectra. Complete structural information can be obtained from 5 to 50 ng of lipid.
AB - In recent years electrospray has become the most popular ionization technique for the analysis of biomolecules. In the present study, we have compared the electrospray and continuous-flow fast-atom bombardment methods of sample introduction and ionization for the analysis of sulphated and sulphonated lipids by tandem moss spectroscopy. The tandem mass spectra obtained when using the two ionization techniques were similar; however, small differences were evident. These differences indicate that the precursor ions generated by fast-atom bombardment and electrospray have different internal energies. The two ionization techniques give similar sensitivities for the recording of collision-induced dissociation spectra. Complete structural information can be obtained from 5 to 50 ng of lipid.
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U2 - 10.1002/(SICI)1097-0231(19960731)10:10<1169::AID-RCM632>3.0.CO;2-M
DO - 10.1002/(SICI)1097-0231(19960731)10:10<1169::AID-RCM632>3.0.CO;2-M
M3 - Article
AN - SCOPUS:0029762192
SN - 0951-4198
VL - 10
SP - 1169
EP - 1174
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 10
ER -