A comprehensively characterized cell line panel highly representative of clinical ovarian high-grade serous carcinomas

Kelsie L. Thu; Mahboubeh Papari-Zareei; Victor Stastny; Kai Song; Michael Peyton; Victor D. Martinez; Yu An Zhang; Isabel B. Castro; Marileila Varella-Garcia; Hanquan Liang; Chao Xing; Ralf Kittler; Sara Milchgrub; Diego H. Castrillon; Heather L. Davidson; C. Patrick Reynolds; Wan L. Lam; Jayanthi Lea; Adi F. Gazdar

doi:10.18632/oncotarget.9929

A comprehensively characterized cell line panel highly representative of clinical ovarian high-grade serous carcinomas

Kelsie L. Thu, Mahboubeh Papari-Zareei, Victor Stastny, Kai Song, Michael Peyton, Victor D. Martinez, Yu An Zhang, Isabel B. Castro, Marileila Varella-Garcia, Hanquan Liang, Chao Xing, Ralf Kittler, Sara Milchgrub, Diego H. Castrillon, Heather L. Davidson, C. Patrick Reynolds, Wan L. Lam, Jayanthi Lea, Adi F. Gazdar

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Recent literature suggests that most widely used ovarian cancer (OVCA) cell models do not recapitulate the molecular features of clinical tumors. To address this limitation, we generated 18 cell lines and 3 corresponding patient-derived xenografts predominantly from high-grade serous carcinoma (HGSOC) peritoneal effusions. Comprehensive genomic characterization and comparison of each model to its parental tumor demonstrated a high degree of molecular similarity. Our characterization included whole exome-sequencing and copy number profiling for cell lines, xenografts, and matched non-malignant tissues, and DNA methylation, gene expression, and spectral karyotyping for a subset of specimens. Compared to the Cancer Genome Atlas (TCGA), our models more closely resembled HGSOC than any other tumor type, justifying their validity as OVCA models. Our meticulously characterized models provide a crucial resource for the OVCA research community that will advance translational findings and ultimately lead to clinical applications.

Original language	English
Pages (from-to)	50489-50499
Number of pages	11
Journal	Oncotarget
Volume	8
Issue number	31
DOIs	https://doi.org/10.18632/oncotarget.9929
Publication status	Published - Feb 7 2017
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Luc Girard and Emily Vucic for their assistance, and The Cancer Genome Atlas consortium for access to their datasets. This work was supported by grants from the Cancer Prevention Research Institute of Texas (RP10763-P1), Canadian Institutes of Health Research (CIHR, FRN 123273, FRN 110949), the Clinical and Translational Science Awards, National Institutes of Health (UL1TR001105, NHLB1R25HL103286 and NCI-CCSG P30 CA046934), scholarships and fellowships from Vanier Canada and CIHR, and a Private Donor

Publisher Copyright:
© Thu et al.

ASJC Scopus Subject Areas

Oncology

PubMed: MeSH publication types

Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncotarget.9929

Cite this

Thu, K. L., Papari-Zareei, M., Stastny, V., Song, K., Peyton, M., Martinez, V. D., Zhang, Y. A., Castro, I. B., Varella-Garcia, M., Liang, H., Xing, C., Kittler, R., Milchgrub, S., Castrillon, D. H., Davidson, H. L., Reynolds, C. P., Lam, W. L., Lea, J., & Gazdar, A. F. (2017). A comprehensively characterized cell line panel highly representative of clinical ovarian high-grade serous carcinomas. Oncotarget, 8(31), 50489-50499. https://doi.org/10.18632/oncotarget.9929

Thu, KL, Papari-Zareei, M, Stastny, V, Song, K, Peyton, M, Martinez, VD, Zhang, YA, Castro, IB, Varella-Garcia, M, Liang, H, Xing, C, Kittler, R, Milchgrub, S, Castrillon, DH, Davidson, HL, Reynolds, CP, Lam, WL, Lea, J & Gazdar, AF 2017, 'A comprehensively characterized cell line panel highly representative of clinical ovarian high-grade serous carcinomas', Oncotarget, vol. 8, no. 31, pp. 50489-50499. https://doi.org/10.18632/oncotarget.9929

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abstract = "Recent literature suggests that most widely used ovarian cancer (OVCA) cell models do not recapitulate the molecular features of clinical tumors. To address this limitation, we generated 18 cell lines and 3 corresponding patient-derived xenografts predominantly from high-grade serous carcinoma (HGSOC) peritoneal effusions. Comprehensive genomic characterization and comparison of each model to its parental tumor demonstrated a high degree of molecular similarity. Our characterization included whole exome-sequencing and copy number profiling for cell lines, xenografts, and matched non-malignant tissues, and DNA methylation, gene expression, and spectral karyotyping for a subset of specimens. Compared to the Cancer Genome Atlas (TCGA), our models more closely resembled HGSOC than any other tumor type, justifying their validity as OVCA models. Our meticulously characterized models provide a crucial resource for the OVCA research community that will advance translational findings and ultimately lead to clinical applications.",

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AU - Peyton, Michael

AU - Martinez, Victor D.

AU - Zhang, Yu An

AU - Castro, Isabel B.

AU - Varella-Garcia, Marileila

AU - Liang, Hanquan

AU - Xing, Chao

AU - Kittler, Ralf

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AU - Lea, Jayanthi

AU - Gazdar, Adi F.

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A comprehensively characterized cell line panel highly representative of clinical ovarian high-grade serous carcinomas

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

UN SDGs

Access to Document

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