A dermal niche for multipotent adult skin-derived precursor cells

Karl J.L. Fernandes; Ian A. McKenzie; Pleasantine Mill; Kristen M. Smith; Mahnaz Akhavan; Fanie Barnabé-Heider; Jeff Biernaskie; Adrienne Junek; Nao R. Kobayashi; Jean G. Toma; David R. Kaplan; Patricia A. Labosky; Victor Rafuse; Chi Chung Hui; Freda D. Miller

doi:10.1038/ncb1181

A dermal niche for multipotent adult skin-derived precursor cells

Karl J.L. Fernandes, Ian A. McKenzie, Pleasantine Mill, Kristen M. Smith, Mahnaz Akhavan, Fanie Barnabé-Heider, Jeff Biernaskie, Adrienne Junek, Nao R. Kobayashi, Jean G. Toma, David R. Kaplan, Patricia A. Labosky, Victor Rafuse, Chi Chung Hui, Freda D. Miller

Medicine

Research output: Contribution to journal › Article › peer-review

660 Citations (Scopus)

Abstract

A fundamental question in stem cell research is whether cultured multipotent adult stem cells represent endogenous multipotent precursor cells. Here we address this question, focusing on SKPs, a cultured adult stem cell from the dermis that generates both neural and mesodermal progeny. We show that SKPs derive from endogenous adult dermal precursors that exhibit properties similar to embryonic neural-crest stem cells. We demonstrate that these endogenous SKPs can first be isolated from skin during embryogenesis and that they persist into adulthood, with a niche in the papillae of hair and whisker follicles. Furthermore, lineage analysis indicates that both hair and whisker follicle dermal papillae contain neural-crest-derived cells, and that SKPs from the whisker pad are of neural-crest origin. We propose that SKPs represent an endogenous embryonic precursor cell that arises in peripheral tissues such as skin during development and maintains multipotency into adulthood.

Original language	English
Pages (from-to)	1082-1093
Number of pages	12
Journal	Nature Cell Biology
Volume	6
Issue number	11
DOIs	https://doi.org/10.1038/ncb1181
Publication status	Published - Nov 2004

Bibliographical note

Funding Information:
We thank A. Aumont, Y. Wang-Ninio, A. Rioux-Taché for technical support; the Miller and Kaplan laboratories for valuable discussion of this work; and A. Nagy for providing mice. This work was funded by grants from The Canadian Stem Cell Network and the Canadian Institutes of Health Resesarch (CIHR) to F.D.M., from CIHR to C.C.H., from the National Institutes of Health to P.A.L., from the National Sciences and Engineering Council (NSERC) to V.R. and the National Cancer Institute of Canada to D.R.K.. K.J.L.F. was funded by fellowships from the CIHR/Canadian Neurotrauma Research Program and Restracomp, K.M.S. from CIHR and Restracomp, F.B.H. from CIHR, J.B. from the Parkinsons Foundation of Canada and N.R.K. from the Christopher Reeves Paralysis Foundation.

ASJC Scopus Subject Areas

Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

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10.1038/ncb1181

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Fernandes, K. J. L., McKenzie, I. A., Mill, P., Smith, K. M., Akhavan, M., Barnabé-Heider, F., Biernaskie, J., Junek, A., Kobayashi, N. R., Toma, J. G., Kaplan, D. R., Labosky, P. A., Rafuse, V., Hui, C. C., & Miller, F. D. (2004). A dermal niche for multipotent adult skin-derived precursor cells. Nature Cell Biology, 6(11), 1082-1093. https://doi.org/10.1038/ncb1181

@article{f0aab7e2f03b4a98ac86d7a13ded632d,

title = "A dermal niche for multipotent adult skin-derived precursor cells",

abstract = "A fundamental question in stem cell research is whether cultured multipotent adult stem cells represent endogenous multipotent precursor cells. Here we address this question, focusing on SKPs, a cultured adult stem cell from the dermis that generates both neural and mesodermal progeny. We show that SKPs derive from endogenous adult dermal precursors that exhibit properties similar to embryonic neural-crest stem cells. We demonstrate that these endogenous SKPs can first be isolated from skin during embryogenesis and that they persist into adulthood, with a niche in the papillae of hair and whisker follicles. Furthermore, lineage analysis indicates that both hair and whisker follicle dermal papillae contain neural-crest-derived cells, and that SKPs from the whisker pad are of neural-crest origin. We propose that SKPs represent an endogenous embryonic precursor cell that arises in peripheral tissues such as skin during development and maintains multipotency into adulthood.",

author = "Fernandes, {Karl J.L.} and McKenzie, {Ian A.} and Pleasantine Mill and Smith, {Kristen M.} and Mahnaz Akhavan and Fanie Barnab{\'e}-Heider and Jeff Biernaskie and Adrienne Junek and Kobayashi, {Nao R.} and Toma, {Jean G.} and Kaplan, {David R.} and Labosky, {Patricia A.} and Victor Rafuse and Hui, {Chi Chung} and Miller, {Freda D.}",

note = "Funding Information: We thank A. Aumont, Y. Wang-Ninio, A. Rioux-Tach{\'e} for technical support; the Miller and Kaplan laboratories for valuable discussion of this work; and A. Nagy for providing mice. This work was funded by grants from The Canadian Stem Cell Network and the Canadian Institutes of Health Resesarch (CIHR) to F.D.M., from CIHR to C.C.H., from the National Institutes of Health to P.A.L., from the National Sciences and Engineering Council (NSERC) to V.R. and the National Cancer Institute of Canada to D.R.K.. K.J.L.F. was funded by fellowships from the CIHR/Canadian Neurotrauma Research Program and Restracomp, K.M.S. from CIHR and Restracomp, F.B.H. from CIHR, J.B. from the Parkinsons Foundation of Canada and N.R.K. from the Christopher Reeves Paralysis Foundation.",

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AU - Fernandes, Karl J.L.

AU - McKenzie, Ian A.

AU - Mill, Pleasantine

AU - Smith, Kristen M.

AU - Akhavan, Mahnaz

AU - Barnabé-Heider, Fanie

AU - Biernaskie, Jeff

AU - Junek, Adrienne

AU - Kobayashi, Nao R.

AU - Toma, Jean G.

AU - Kaplan, David R.

AU - Labosky, Patricia A.

AU - Rafuse, Victor

AU - Hui, Chi Chung

AU - Miller, Freda D.

N1 - Funding Information: We thank A. Aumont, Y. Wang-Ninio, A. Rioux-Taché for technical support; the Miller and Kaplan laboratories for valuable discussion of this work; and A. Nagy for providing mice. This work was funded by grants from The Canadian Stem Cell Network and the Canadian Institutes of Health Resesarch (CIHR) to F.D.M., from CIHR to C.C.H., from the National Institutes of Health to P.A.L., from the National Sciences and Engineering Council (NSERC) to V.R. and the National Cancer Institute of Canada to D.R.K.. K.J.L.F. was funded by fellowships from the CIHR/Canadian Neurotrauma Research Program and Restracomp, K.M.S. from CIHR and Restracomp, F.B.H. from CIHR, J.B. from the Parkinsons Foundation of Canada and N.R.K. from the Christopher Reeves Paralysis Foundation.

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N2 - A fundamental question in stem cell research is whether cultured multipotent adult stem cells represent endogenous multipotent precursor cells. Here we address this question, focusing on SKPs, a cultured adult stem cell from the dermis that generates both neural and mesodermal progeny. We show that SKPs derive from endogenous adult dermal precursors that exhibit properties similar to embryonic neural-crest stem cells. We demonstrate that these endogenous SKPs can first be isolated from skin during embryogenesis and that they persist into adulthood, with a niche in the papillae of hair and whisker follicles. Furthermore, lineage analysis indicates that both hair and whisker follicle dermal papillae contain neural-crest-derived cells, and that SKPs from the whisker pad are of neural-crest origin. We propose that SKPs represent an endogenous embryonic precursor cell that arises in peripheral tissues such as skin during development and maintains multipotency into adulthood.

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A dermal niche for multipotent adult skin-derived precursor cells

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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