A new method for quantifying iodine in a starch-iodine matrix

Bruce A. Manion; Bruce E. Holbein; Massimo F. Marcone; Koushik Seetharaman

doi:10.1016/j.carres.2010.09.025

A new method for quantifying iodine in a starch-iodine matrix

Bruce A. Manion, Bruce E. Holbein, Massimo F. Marcone, Koushik Seetharaman

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A rapid and sensitive method for quantifying iodine in intact starch granules using gas chromatography is described with detection limits as low as 0.2% (w/w) iodine in starch. Sample preparation includes NaBH₄ reduction of the various iodine species associated with starch to the colorless soluble iodide ion, followed by its quantitative derivatization to EtI using Et3O+BF4- in CH₂Cl₂. Identification and quantification of EtI is carried out by extraction and injection of the EtI so generated in CH₂Cl₂ into a gas chromatography-mass spectrometer (GC-MS). Routine quantification of EtI was then performed using GC with a flame ionization detector (GC-FID). Results for different iodine:potassium iodide ratios of the initially bound iodine and for seven different starch matrices showed that in all cases regression coefficients for the standards were high (R² >0.96).

Original language	English
Pages (from-to)	2698-2704
Number of pages	7
Journal	Carbohydrate Research
Volume	345
Issue number	18
DOIs	https://doi.org/10.1016/j.carres.2010.09.025
Publication status	Published - Dec 10 2010
Externally published	Yes

ASJC Scopus Subject Areas

Analytical Chemistry
Biochemistry
Organic Chemistry

PubMed: MeSH publication types

Journal Article

Access to Document

10.1016/j.carres.2010.09.025

Cite this

@article{04d262ff20b24d00b0f87fa6c68de47f,

title = "A new method for quantifying iodine in a starch-iodine matrix",

abstract = "A rapid and sensitive method for quantifying iodine in intact starch granules using gas chromatography is described with detection limits as low as 0.2% (w/w) iodine in starch. Sample preparation includes NaBH4 reduction of the various iodine species associated with starch to the colorless soluble iodide ion, followed by its quantitative derivatization to EtI using Et3O+BF4- in CH2Cl2. Identification and quantification of EtI is carried out by extraction and injection of the EtI so generated in CH2Cl2 into a gas chromatography-mass spectrometer (GC-MS). Routine quantification of EtI was then performed using GC with a flame ionization detector (GC-FID). Results for different iodine:potassium iodide ratios of the initially bound iodine and for seven different starch matrices showed that in all cases regression coefficients for the standards were high (R2 >0.96).",

author = "Manion, {Bruce A.} and Holbein, {Bruce E.} and Marcone, {Massimo F.} and Koushik Seetharaman",

year = "2010",

month = dec,

day = "10",

doi = "10.1016/j.carres.2010.09.025",

language = "English",

volume = "345",

pages = "2698--2704",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier BV",

number = "18",

}

TY - JOUR

T1 - A new method for quantifying iodine in a starch-iodine matrix

AU - Manion, Bruce A.

AU - Holbein, Bruce E.

AU - Marcone, Massimo F.

AU - Seetharaman, Koushik

PY - 2010/12/10

Y1 - 2010/12/10

N2 - A rapid and sensitive method for quantifying iodine in intact starch granules using gas chromatography is described with detection limits as low as 0.2% (w/w) iodine in starch. Sample preparation includes NaBH4 reduction of the various iodine species associated with starch to the colorless soluble iodide ion, followed by its quantitative derivatization to EtI using Et3O+BF4- in CH2Cl2. Identification and quantification of EtI is carried out by extraction and injection of the EtI so generated in CH2Cl2 into a gas chromatography-mass spectrometer (GC-MS). Routine quantification of EtI was then performed using GC with a flame ionization detector (GC-FID). Results for different iodine:potassium iodide ratios of the initially bound iodine and for seven different starch matrices showed that in all cases regression coefficients for the standards were high (R2 >0.96).

AB - A rapid and sensitive method for quantifying iodine in intact starch granules using gas chromatography is described with detection limits as low as 0.2% (w/w) iodine in starch. Sample preparation includes NaBH4 reduction of the various iodine species associated with starch to the colorless soluble iodide ion, followed by its quantitative derivatization to EtI using Et3O+BF4- in CH2Cl2. Identification and quantification of EtI is carried out by extraction and injection of the EtI so generated in CH2Cl2 into a gas chromatography-mass spectrometer (GC-MS). Routine quantification of EtI was then performed using GC with a flame ionization detector (GC-FID). Results for different iodine:potassium iodide ratios of the initially bound iodine and for seven different starch matrices showed that in all cases regression coefficients for the standards were high (R2 >0.96).

UR - http://www.scopus.com/inward/record.url?scp=78649325834&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649325834&partnerID=8YFLogxK

U2 - 10.1016/j.carres.2010.09.025

DO - 10.1016/j.carres.2010.09.025

M3 - Article

C2 - 20971452

AN - SCOPUS:78649325834

SN - 0008-6215

VL - 345

SP - 2698

EP - 2704

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 18

ER -

A new method for quantifying iodine in a starch-iodine matrix

Abstract

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

Other files and links

Fingerprint

Cite this