A new simple tubular flow cell for use with variable angle spectroscopic ellipsometry: A high throughput in situ protein adsorption study

T. M. Byrne; S. Trussler; M. A. McArthur; L. B. Lohstreter; Zhijun Bai; M. J. Filiaggi; J. R. Dahn

doi:10.1016/j.susc.2009.07.037

A new simple tubular flow cell for use with variable angle spectroscopic ellipsometry: A high throughput in situ protein adsorption study

T. M. Byrne, S. Trussler, M. A. McArthur, L. B. Lohstreter, Zhijun Bai, M. J. Filiaggi, J. R. Dahn

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A simple and novel flow cell design is presented here for use with variable angle spectroscopic ellipsometry (VASE) to study the adsorption of liquid-borne species on reflective surfaces. The flow cell allows a sample as large as 6 mm × 75 mm to be probed point by point and at any common ellipsometric angle of incidence, unlike other designs. Using our flow cell system with VASE, combinatorial films of Al_1-xNb_x, Al_1-xTa_x, and Al_1-xTi_x (0 ≤ x ≤ 1) were tested in situ for fibrinogen affinity along their 75 mm long compositional gradients. Fibrinogen adsorption on the films was found to be closely correlated to the various surface oxide fractions, with high alumina content at the surface leading to low amounts of adsorbed fibrinogen for each binary library. Adsorbed amounts measured in situ were in agreement with previously obtained values found using ex situ techniques.

Original language	English
Pages (from-to)	2888-2895
Number of pages	8
Journal	Surface Science
Volume	603
Issue number	18
DOIs	https://doi.org/10.1016/j.susc.2009.07.037
Publication status	Published - Sept 15 2009

Bibliographical note

Funding Information:
The financial support of Medtronic, Inc. (USA) is greatly acknowledged.

ASJC Scopus Subject Areas

Condensed Matter Physics
Surfaces and Interfaces
Surfaces, Coatings and Films
Materials Chemistry

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10.1016/j.susc.2009.07.037

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title = "A new simple tubular flow cell for use with variable angle spectroscopic ellipsometry: A high throughput in situ protein adsorption study",

abstract = "A simple and novel flow cell design is presented here for use with variable angle spectroscopic ellipsometry (VASE) to study the adsorption of liquid-borne species on reflective surfaces. The flow cell allows a sample as large as 6 mm × 75 mm to be probed point by point and at any common ellipsometric angle of incidence, unlike other designs. Using our flow cell system with VASE, combinatorial films of Al1-xNbx, Al1-xTax, and Al1-xTix (0 ≤ x ≤ 1) were tested in situ for fibrinogen affinity along their 75 mm long compositional gradients. Fibrinogen adsorption on the films was found to be closely correlated to the various surface oxide fractions, with high alumina content at the surface leading to low amounts of adsorbed fibrinogen for each binary library. Adsorbed amounts measured in situ were in agreement with previously obtained values found using ex situ techniques.",

author = "Byrne, {T. M.} and S. Trussler and McArthur, {M. A.} and Lohstreter, {L. B.} and Zhijun Bai and Filiaggi, {M. J.} and Dahn, {J. R.}",

note = "Funding Information: The financial support of Medtronic, Inc. (USA) is greatly acknowledged.",

year = "2009",

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doi = "10.1016/j.susc.2009.07.037",

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T2 - A high throughput in situ protein adsorption study

AU - Byrne, T. M.

AU - Trussler, S.

AU - McArthur, M. A.

AU - Lohstreter, L. B.

AU - Bai, Zhijun

AU - Filiaggi, M. J.

AU - Dahn, J. R.

N1 - Funding Information: The financial support of Medtronic, Inc. (USA) is greatly acknowledged.

PY - 2009/9/15

Y1 - 2009/9/15

N2 - A simple and novel flow cell design is presented here for use with variable angle spectroscopic ellipsometry (VASE) to study the adsorption of liquid-borne species on reflective surfaces. The flow cell allows a sample as large as 6 mm × 75 mm to be probed point by point and at any common ellipsometric angle of incidence, unlike other designs. Using our flow cell system with VASE, combinatorial films of Al1-xNbx, Al1-xTax, and Al1-xTix (0 ≤ x ≤ 1) were tested in situ for fibrinogen affinity along their 75 mm long compositional gradients. Fibrinogen adsorption on the films was found to be closely correlated to the various surface oxide fractions, with high alumina content at the surface leading to low amounts of adsorbed fibrinogen for each binary library. Adsorbed amounts measured in situ were in agreement with previously obtained values found using ex situ techniques.

AB - A simple and novel flow cell design is presented here for use with variable angle spectroscopic ellipsometry (VASE) to study the adsorption of liquid-borne species on reflective surfaces. The flow cell allows a sample as large as 6 mm × 75 mm to be probed point by point and at any common ellipsometric angle of incidence, unlike other designs. Using our flow cell system with VASE, combinatorial films of Al1-xNbx, Al1-xTax, and Al1-xTix (0 ≤ x ≤ 1) were tested in situ for fibrinogen affinity along their 75 mm long compositional gradients. Fibrinogen adsorption on the films was found to be closely correlated to the various surface oxide fractions, with high alumina content at the surface leading to low amounts of adsorbed fibrinogen for each binary library. Adsorbed amounts measured in situ were in agreement with previously obtained values found using ex situ techniques.

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A new simple tubular flow cell for use with variable angle spectroscopic ellipsometry: A high throughput in situ protein adsorption study

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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