A novel protein from ectocarpus sp. Improves salinity and high temperature stress tolerance in arabidopsis thaliana

Pramod Rathor; Tudor Borza; Sophia Stone; Thierry Tonon; Svetlana Yurgel; Philippe Potin; Balakrishnan Prithiviraj

doi:10.3390/ijms22041971

A novel protein from ectocarpus sp. Improves salinity and high temperature stress tolerance in arabidopsis thaliana

Pramod Rathor, Tudor Borza, Sophia Stone, Thierry Tonon, Svetlana Yurgel, Philippe Potin, Balakrishnan Prithiviraj

Medicine

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Brown alga Ectocarpus sp. belongs to Phaeophyceae, a class of macroalgae that evolved complex multicellularity. Ectocarpus sp. is a dominant seaweed in temperate regions, abundant mostly in the intertidal zones, an environment with high levels of abiotic stresses. Previous transcriptomic analysis of Ectocarpus sp. revealed several genes consistently induced by various abiotic stresses; one of these genes is Esi0017_0056, which encodes a protein with unknown function. Bioinformatics analyses indicated that the protein encoded by Esi0017_0056 is soluble and monomeric. The protein was successfully expressed in Escherichia coli, Arabidopsis thaliana and Nicotiana benthamiana. In A. thaliana the gene was expressed under constitutive and stress inducible promoters which led to improved tolerance to high salinity and temperature stresses. The expression of several key abiotic stress-related genes was studied in transgenic and wild type A. thaliana by qPCR. Expression analysis revealed that genes involved in ABA-induced abiotic stress tolerance, K⁺ homeostasis, and chaperon activities were significantly up-regulated in the transgenic line. This study is the first report in which an unknown function Ectocarpus sp. gene, highly responsive to abiotic stresses, was successfully expressed in A. thaliana, leading to improved tolerance to salt and temperature stress.

Original language	English
Article number	1971
Pages (from-to)	1-20
Number of pages	20
Journal	International Journal of Molecular Sciences
Volume	22
Issue number	4
DOIs	https://doi.org/10.3390/ijms22041971
Publication status	Published - Feb 2 2021

Bibliographical note

Funding Information:
Funding: BP’s lab was supported by NSERC-DG grant 1177546, MITACS and France-Canada Research Fund (FCRF). PR was supported by an NSERC Industrial Postgraduate Scholarship and a MITACS Globalink Research Award. PP and TT were also supported by the Agence Nationale de la Recherche via the investment expenditure program IDEALG Grant ANR-10-BTBR-04.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

ASJC Scopus Subject Areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

PubMed: MeSH publication types

Journal Article

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title = "A novel protein from ectocarpus sp. Improves salinity and high temperature stress tolerance in arabidopsis thaliana",

abstract = "Brown alga Ectocarpus sp. belongs to Phaeophyceae, a class of macroalgae that evolved complex multicellularity. Ectocarpus sp. is a dominant seaweed in temperate regions, abundant mostly in the intertidal zones, an environment with high levels of abiotic stresses. Previous transcriptomic analysis of Ectocarpus sp. revealed several genes consistently induced by various abiotic stresses; one of these genes is Esi0017_0056, which encodes a protein with unknown function. Bioinformatics analyses indicated that the protein encoded by Esi0017_0056 is soluble and monomeric. The protein was successfully expressed in Escherichia coli, Arabidopsis thaliana and Nicotiana benthamiana. In A. thaliana the gene was expressed under constitutive and stress inducible promoters which led to improved tolerance to high salinity and temperature stresses. The expression of several key abiotic stress-related genes was studied in transgenic and wild type A. thaliana by qPCR. Expression analysis revealed that genes involved in ABA-induced abiotic stress tolerance, K+ homeostasis, and chaperon activities were significantly up-regulated in the transgenic line. This study is the first report in which an unknown function Ectocarpus sp. gene, highly responsive to abiotic stresses, was successfully expressed in A. thaliana, leading to improved tolerance to salt and temperature stress.",

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AU - Yurgel, Svetlana

AU - Potin, Philippe

AU - Prithiviraj, Balakrishnan

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A novel protein from ectocarpus sp. Improves salinity and high temperature stress tolerance in arabidopsis thaliana

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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