A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels

Marianne Swain; Neil W. Ross

doi:10.1002/elps.11501601159

A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels

Marianne Swain, Neil W. Ross

Research output: Contribution to journal › Article › peer-review

133 Citations (Scopus)

Abstract

A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/acetic acid and prior to glutaraldehyde cross‐linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.

Original language	English
Pages (from-to)	948-951
Number of pages	4
Journal	Electrophoresis
Volume	16
Issue number	1
DOIs	https://doi.org/10.1002/elps.11501601159
Publication status	Published - 1995
Externally published	Yes

ASJC Scopus Subject Areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.11501601159

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title = "A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels",

abstract = "A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/acetic acid and prior to glutaraldehyde cross‐linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.",

author = "Marianne Swain and Ross, {Neil W.}",

year = "1995",

doi = "10.1002/elps.11501601159",

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AU - Swain, Marianne

AU - Ross, Neil W.

PY - 1995

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AB - A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/acetic acid and prior to glutaraldehyde cross‐linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.

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DO - 10.1002/elps.11501601159

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C2 - 7498141

AN - SCOPUS:0029075044

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JO - Electrophoresis

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IS - 1

ER -

A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels

Abstract

ASJC Scopus Subject Areas

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