Absolute quantification of Atlantic salmon and rainbow trout vitellogenin by the 'signature peptide' approach using electrospray ionization QqToF tandem mass spectrometry

Alejandro M. Cohen, Atef A.H. Mansour, Joseph H. Banoub

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

In this work, we present a simple method for absolute quantification of plasma vitellogenin from both rainbow trout and Atlantic salmon. Plasma samples obtained from control and β-estradiol induced fish were digested with trypsin. A characteristic 'signature peptide' was selected and analyzed by high performance liquid chromatography (HPLC) coupled to an electrospray quadrupole-time-of-flight tandem mass spectrometer, using a deuterated homolog peptide as an internal standard. The hybrid tandem mass spectrometer was operated in a 'pseudo' selected reaction-monitoring mode in which three diagnostic product ions were monitored for identification and quantification purposes. The reproducibility (coefficient of variation ∼5%) and sensitivity (limit of quantification (LOQ) of 0.009 mg/ml) achieved by this simple assay allow it to be considered as an alternative to immunological assays.

Original languageEnglish
Pages (from-to)646-658
Number of pages13
JournalJournal of Mass Spectrometry
Volume41
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

ASJC Scopus Subject Areas

  • Spectroscopy

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