Abstract
In this work, we present a simple method for absolute quantification of plasma vitellogenin from both rainbow trout and Atlantic salmon. Plasma samples obtained from control and β-estradiol induced fish were digested with trypsin. A characteristic 'signature peptide' was selected and analyzed by high performance liquid chromatography (HPLC) coupled to an electrospray quadrupole-time-of-flight tandem mass spectrometer, using a deuterated homolog peptide as an internal standard. The hybrid tandem mass spectrometer was operated in a 'pseudo' selected reaction-monitoring mode in which three diagnostic product ions were monitored for identification and quantification purposes. The reproducibility (coefficient of variation ∼5%) and sensitivity (limit of quantification (LOQ) of 0.009 mg/ml) achieved by this simple assay allow it to be considered as an alternative to immunological assays.
Original language | English |
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Pages (from-to) | 646-658 |
Number of pages | 13 |
Journal | Journal of Mass Spectrometry |
Volume | 41 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2006 |
Externally published | Yes |
ASJC Scopus Subject Areas
- Spectroscopy