An evaluation of the contribution of membrane lipids to protection against ultraviolet radiation

Robert Anderson; Maleki Daya; Jeff Reeve

doi:10.1016/0005-2736(87)90027-7

An evaluation of the contribution of membrane lipids to protection against ultraviolet radiation

Robert Anderson, Maleki Daya, Jeff Reeve

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Using dioleoylphosphatidylcholine liposomes incorporating various fatty acids and neutral lipids, we have examined the ability of such lipids to provide protection of Escherichia coli and vesicular stomatitis virus (VSV) against the lethal effect of ultraviolet (254 nm) radiation. While the presence of varying amounts of saturated (palmitic) or polyunsaturated (arachidonic) fatty acids or the lipid antioxidant, α-tocopherol, had little effect on killing by ultraviolet radiation, considerable radioprotection was observed with β-carotene, retinal and vitamin K-1 at final concentrations of 1 mg/ml. In another approach, vesicular stomatitis virus grown under conditions in which its envelope fatty acid composition was substantially modified, showed little change in its sensitivity to inactivation by ultraviolet radiation. The results provide strong evidence for a radioprotective role of certain, relatively rare natural lipid components with conjugated polyene systems, but not of the more ubiquitous and abundant membrane fatty acids.

Original language	English
Pages (from-to)	227-230
Number of pages	4
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	905
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(87)90027-7
Publication status	Published - Nov 27 1987
Externally published	Yes

Bibliographical note

Funding Information:
Taken together,t he results of the above studies strongly argue that biologicalk illing by ultraviolet radiation may be significantlym oderatedb y the presenceo f relativelyr are membraneli pid components, such as fl-carotene,r etinal or vitamin K, while the more ubiquitous and abundant membrane fatty acids have a negligiblec ontribution.It is also clear that compounds (e.g. c~-tocopherol) which are effectivei nhibitorsof lipid peroxidation \[23,24\]d o not necessarily provide protection against oxidative cell-killing processesg enerated during ultravioleti rradiation. The support of the Medical Research Council of Canada is gratefully acknowledgedR. .A. is a Scholar of the Alberta Heritage Foundation for Medical Research.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0005-2736(87)90027-7

Cite this

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title = "An evaluation of the contribution of membrane lipids to protection against ultraviolet radiation",

abstract = "Using dioleoylphosphatidylcholine liposomes incorporating various fatty acids and neutral lipids, we have examined the ability of such lipids to provide protection of Escherichia coli and vesicular stomatitis virus (VSV) against the lethal effect of ultraviolet (254 nm) radiation. While the presence of varying amounts of saturated (palmitic) or polyunsaturated (arachidonic) fatty acids or the lipid antioxidant, α-tocopherol, had little effect on killing by ultraviolet radiation, considerable radioprotection was observed with β-carotene, retinal and vitamin K-1 at final concentrations of 1 mg/ml. In another approach, vesicular stomatitis virus grown under conditions in which its envelope fatty acid composition was substantially modified, showed little change in its sensitivity to inactivation by ultraviolet radiation. The results provide strong evidence for a radioprotective role of certain, relatively rare natural lipid components with conjugated polyene systems, but not of the more ubiquitous and abundant membrane fatty acids.",

author = "Robert Anderson and Maleki Daya and Jeff Reeve",

note = "Funding Information: Taken together,t he results of the above studies strongly argue that biologicalk illing by ultraviolet radiation may be significantlym oderatedb y the presenceo f relativelyr are membraneli pid components, such as fl-carotene,r etinal or vitamin K, while the more ubiquitous and abundant membrane fatty acids have a negligiblec ontribution.It is also clear that compounds (e.g. c~-tocopherol) which are effectivei nhibitorsof lipid peroxidation \[23,24\]d o not necessarily provide protection against oxidative cell-killing processesg enerated during ultravioleti rradiation. The support of the Medical Research Council of Canada is gratefully acknowledgedR. .A. is a Scholar of the Alberta Heritage Foundation for Medical Research.",

year = "1987",

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doi = "10.1016/0005-2736(87)90027-7",

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AU - Reeve, Jeff

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N2 - Using dioleoylphosphatidylcholine liposomes incorporating various fatty acids and neutral lipids, we have examined the ability of such lipids to provide protection of Escherichia coli and vesicular stomatitis virus (VSV) against the lethal effect of ultraviolet (254 nm) radiation. While the presence of varying amounts of saturated (palmitic) or polyunsaturated (arachidonic) fatty acids or the lipid antioxidant, α-tocopherol, had little effect on killing by ultraviolet radiation, considerable radioprotection was observed with β-carotene, retinal and vitamin K-1 at final concentrations of 1 mg/ml. In another approach, vesicular stomatitis virus grown under conditions in which its envelope fatty acid composition was substantially modified, showed little change in its sensitivity to inactivation by ultraviolet radiation. The results provide strong evidence for a radioprotective role of certain, relatively rare natural lipid components with conjugated polyene systems, but not of the more ubiquitous and abundant membrane fatty acids.

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An evaluation of the contribution of membrane lipids to protection against ultraviolet radiation

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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