Antibacterial activities of physiologically stable, self-assembled peptide nanoparticles

Nauman Nazeer; Jeffrey R. Simmons; Jan K. Rainey; Juan Carlos Rodriguez-Lecompte; Marya Ahmed

doi:10.1039/d1tb01864g

Antibacterial activities of physiologically stable, self-assembled peptide nanoparticles

Nauman Nazeer, Jeffrey R. Simmons, Jan K. Rainey, Juan Carlos Rodriguez-Lecompte, Marya Ahmed

Medicine

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

In this study, we report that host defense protein-derived ten amino acid long disulfide-linked peptides self-assemble in the form of β-sheets and β-turns, and exhibit concentration-dependent self-assembly in the form of nanospheres, termed as disulfide linked nanospheres (DSNs). As expected, bare DSNs are prone to aggregation in ionic solutions and in the presence of serum proteins. To yield physiologically stable self-assembled peptide-based materials, DSNs are stabilized in the form of supramolecular assemblies using β-cyclodextrins (β-CD) and fucoidan, as delivery carriers. The inclusion complexes of DSNs with β-CD (β-CD-DSN) and electrostatic complexation of fucoidan with DSNs (FC-DSN) stabilizes the secondary structure of DSNs. Comparison of β-CD-DSNs with FC-DSNs reveals that inclusion complexes of DSNs formed in the presence of β-CD are highly stable under physiological conditions, show high cellular uptake, exhibit bacterial flocculation, and enhance antibacterial efficacies of DSNs in a range of Gram-positive and Gram-negative bacteria. This journal is

Original language	English
Pages (from-to)	9041-9054
Number of pages	14
Journal	Journal of Materials Chemistry B
Volume	9
Issue number	43
DOIs	https://doi.org/10.1039/d1tb01864g
Publication status	Published - Nov 21 2021

Bibliographical note

Funding Information:
This work was supported by a National Sciences and Engineering Research Council of Canada (NSERC) Discovery Grants (RGPIN/05907-2017, RGPIN-06183/2018), an NSERC Alexander Graham Bell Canada Graduate Scholarship-Doctoral (CGSD3 – 547561 – 2020), the NSERC CREATE Training Program in BioActives (510963), Canadian Poultry Research Council (CPRC), grant and a Nova Scotia Graduate Scholarship. Equipment and infrastructure were obtained through grants from NSERC, Atlantic Canada Opportunity Agency (ACOA), innovation PEI, the Canadian Foundation for Innovation, and the Dalhousie Medical Research Foundation.

Publisher Copyright:
© The Royal Society of Chemistry.

ASJC Scopus Subject Areas

General Chemistry
Biomedical Engineering
General Materials Science

PubMed: MeSH publication types

Journal Article

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10.1039/d1tb01864g

Cite this

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abstract = "In this study, we report that host defense protein-derived ten amino acid long disulfide-linked peptides self-assemble in the form of β-sheets and β-turns, and exhibit concentration-dependent self-assembly in the form of nanospheres, termed as disulfide linked nanospheres (DSNs). As expected, bare DSNs are prone to aggregation in ionic solutions and in the presence of serum proteins. To yield physiologically stable self-assembled peptide-based materials, DSNs are stabilized in the form of supramolecular assemblies using β-cyclodextrins (β-CD) and fucoidan, as delivery carriers. The inclusion complexes of DSNs with β-CD (β-CD-DSN) and electrostatic complexation of fucoidan with DSNs (FC-DSN) stabilizes the secondary structure of DSNs. Comparison of β-CD-DSNs with FC-DSNs reveals that inclusion complexes of DSNs formed in the presence of β-CD are highly stable under physiological conditions, show high cellular uptake, exhibit bacterial flocculation, and enhance antibacterial efficacies of DSNs in a range of Gram-positive and Gram-negative bacteria. This journal is ",

author = "Nauman Nazeer and Simmons, {Jeffrey R.} and Rainey, {Jan K.} and Rodriguez-Lecompte, {Juan Carlos} and Marya Ahmed",

note = "Funding Information: This work was supported by a National Sciences and Engineering Research Council of Canada (NSERC) Discovery Grants (RGPIN/05907-2017, RGPIN-06183/2018), an NSERC Alexander Graham Bell Canada Graduate Scholarship-Doctoral (CGSD3 – 547561 – 2020), the NSERC CREATE Training Program in BioActives (510963), Canadian Poultry Research Council (CPRC), grant and a Nova Scotia Graduate Scholarship. Equipment and infrastructure were obtained through grants from NSERC, Atlantic Canada Opportunity Agency (ACOA), innovation PEI, the Canadian Foundation for Innovation, and the Dalhousie Medical Research Foundation. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

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Antibacterial activities of physiologically stable, self-assembled peptide nanoparticles

Abstract

Bibliographical note

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PubMed: MeSH publication types

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