Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species

Liisa A. Tremere; Raphael Pinaud; Jens Grosche; Wolfgang Härtig; Douglas D. Rasmusson

doi:10.1097/00001756-200007140-00023

Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species

Liisa A. Tremere, Raphael Pinaud, Jens Grosche, Wolfgang Härtig, Douglas D. Rasmusson

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

192-IgG is an antibody directed against the p75 low affinity nerve growth factor receptor in rats, whereas ME 20.4 was raised against the analogous protein in humans. Coupled to saporin, 192-IgG and ME 20.4 have been used to lesion basal forebrain neurons in rats and primates, respectively. We compared the cross-reactivity of 192-IgG and ME 20.4 in the basal forebrain of rat, human, dog, cat, raccoon, pig, and rabbit. We found excellent species cross-reactivity of ME 20.4 in dog, raccoon, cat, pig and rabbit. In contrast, 192-IgG did not label neurons in any species other than rat. Our findings suggest that ME 20.4-saporin could be used to produce cholinergic basal forebrain lesions in several non-primate species. (C) 2000 Lippincott Williams and Wilkins.

Original language	English
Pages (from-to)	2177-2183
Number of pages	7
Journal	NeuroReport
Volume	11
Issue number	10
DOIs	https://doi.org/10.1097/00001756-200007140-00023
Publication status	Published - Jul 14 2000

ASJC Scopus Subject Areas

General Neuroscience

PubMed: MeSH publication types

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

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10.1097/00001756-200007140-00023

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title = "Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species",

abstract = "192-IgG is an antibody directed against the p75 low affinity nerve growth factor receptor in rats, whereas ME 20.4 was raised against the analogous protein in humans. Coupled to saporin, 192-IgG and ME 20.4 have been used to lesion basal forebrain neurons in rats and primates, respectively. We compared the cross-reactivity of 192-IgG and ME 20.4 in the basal forebrain of rat, human, dog, cat, raccoon, pig, and rabbit. We found excellent species cross-reactivity of ME 20.4 in dog, raccoon, cat, pig and rabbit. In contrast, 192-IgG did not label neurons in any species other than rat. Our findings suggest that ME 20.4-saporin could be used to produce cholinergic basal forebrain lesions in several non-primate species. (C) 2000 Lippincott Williams and Wilkins.",

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T1 - Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species

AU - Tremere, Liisa A.

AU - Pinaud, Raphael

AU - Grosche, Jens

AU - Härtig, Wolfgang

AU - Rasmusson, Douglas D.

PY - 2000/7/14

Y1 - 2000/7/14

N2 - 192-IgG is an antibody directed against the p75 low affinity nerve growth factor receptor in rats, whereas ME 20.4 was raised against the analogous protein in humans. Coupled to saporin, 192-IgG and ME 20.4 have been used to lesion basal forebrain neurons in rats and primates, respectively. We compared the cross-reactivity of 192-IgG and ME 20.4 in the basal forebrain of rat, human, dog, cat, raccoon, pig, and rabbit. We found excellent species cross-reactivity of ME 20.4 in dog, raccoon, cat, pig and rabbit. In contrast, 192-IgG did not label neurons in any species other than rat. Our findings suggest that ME 20.4-saporin could be used to produce cholinergic basal forebrain lesions in several non-primate species. (C) 2000 Lippincott Williams and Wilkins.

AB - 192-IgG is an antibody directed against the p75 low affinity nerve growth factor receptor in rats, whereas ME 20.4 was raised against the analogous protein in humans. Coupled to saporin, 192-IgG and ME 20.4 have been used to lesion basal forebrain neurons in rats and primates, respectively. We compared the cross-reactivity of 192-IgG and ME 20.4 in the basal forebrain of rat, human, dog, cat, raccoon, pig, and rabbit. We found excellent species cross-reactivity of ME 20.4 in dog, raccoon, cat, pig and rabbit. In contrast, 192-IgG did not label neurons in any species other than rat. Our findings suggest that ME 20.4-saporin could be used to produce cholinergic basal forebrain lesions in several non-primate species. (C) 2000 Lippincott Williams and Wilkins.

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Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species

Abstract

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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