Abstract
In this study, application of magnetic beads (Dynabeads) coated with Aeromonas salmonicida lipopolysaccharide-specific polyclonal antisera to MS-based characterization of bacterial lipopolysaccharides has been evaluated. The results showed that the affinity-based preconcentration strategy resulted in at least a 100-fold increase in the detection of sensitivity, affording direct capillary electrophoresis (CE)-MS analysis of A. salmonicida lipopolysaccharide O-chain polysaccharide from in vitro-cultured cells. Subsequent CE-MS analysis of in vivo-grown cells of A. salmonicida confirmed significant changes in the structure of the lipopolysaccharide O-chain polysaccharide as a result of in vivo cultivation.
| Original language | English |
|---|---|
| Pages (from-to) | 148-155 |
| Number of pages | 8 |
| Journal | FEMS Microbiology Letters |
| Volume | 295 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 2009 |
ASJC Scopus Subject Areas
- Microbiology
- Molecular Biology
- Genetics
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Wang, Z., Liu, X., Garduño, E., Garduño, R. A., Li, J., & Altman, E. (2009). Application of an immunoaffinity-based preconcentration method for mass spectrometric analysis of the O-chain polysaccharide of Aeromonas salmonicida from in vitro- and in vivo-grown cells. FEMS Microbiology Letters, 295(2), 148-155. https://doi.org/10.1111/j.1574-6968.2009.01612.x