Assembly of a protein "brush" by end-grafting titin fragments to liposomes

Mohamed Chami; Barbara Franke; Olga Mayans; Laurent Kreplak

doi:10.1016/j.jbiosc.2011.04.014

Assembly of a protein "brush" by end-grafting titin fragments to liposomes

Mohamed Chami, Barbara Franke, Olga Mayans, Laurent Kreplak

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Titin is a giant filamentous protein of striated muscle composed of > 300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.

Original language	English
Pages (from-to)	178-179
Number of pages	2
Journal	Journal of Bioscience and Bioengineering
Volume	112
Issue number	2
DOIs	https://doi.org/10.1016/j.jbiosc.2011.04.014
Publication status	Published - Aug 2011

ASJC Scopus Subject Areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2011.04.014

Cite this

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AU - Chami, Mohamed

AU - Franke, Barbara

AU - Mayans, Olga

AU - Kreplak, Laurent

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AB - Titin is a giant filamentous protein of striated muscle composed of > 300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.

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Assembly of a protein "brush" by end-grafting titin fragments to liposomes

Abstract

ASJC Scopus Subject Areas

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