Assembly of a protein "brush" by end-grafting titin fragments to liposomes

Mohamed Chami, Barbara Franke, Olga Mayans, Laurent Kreplak

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Titin is a giant filamentous protein of striated muscle composed of > 300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.

Original languageEnglish
Pages (from-to)178-179
Number of pages2
JournalJournal of Bioscience and Bioengineering
Volume112
Issue number2
DOIs
Publication statusPublished - Aug 2011

ASJC Scopus Subject Areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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