Axotomy affects calcium-sensitive potassium conductance in sympathetic neurones

Melanie E.M. Kelly; Tessa Gordon; Jacob Shapiro; Peter A. Smith

doi:10.1016/0304-3940(86)90391-5

Axotomy affects calcium-sensitive potassium conductance in sympathetic neurones

Melanie E.M. Kelly, Tessa Gordon, Jacob Shapiro, Peter A. Smith

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Following peripheral nerve section, the cell body and proximal axon undergo various physiological and morphological changes which may be associated with regrowth. We found that axotomy reduced the amplitude and duration of the afterhyperpolarization (AHP) of the action potential (AP) of bullfrog sympathetic neurones. In addition, the repolarization of the AP was slowed. The Ca²⁺-channel blocker, Cd²⁺ (200 μM) produced similar effects in normal cells by blocking the Ca²⁺-sensitive K⁺ conductances (g_{k, Ca}) which contribute to the AHP and to AP repolarization. Although Ca²⁺-channel function was preserved after axotomy, Cd²⁺ did not affect axotomized neurones. This suggests that axotomy promotes a functional loss of g_{k, Ca} which could reflect a regenerative response of the cell to injury.

Original language	English
Pages (from-to)	163-168
Number of pages	6
Journal	Neuroscience Letters
Volume	67
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(86)90391-5
Publication status	Published - Jun 18 1986
Externally published	Yes

Bibliographical note

Funding Information:
Supported by grants from the Medical Research Council of Canada and the Alberta Heritage Foundation for Medical Research. We thank Dr. R.B. Stein for his comments on the manuscript.

ASJC Scopus Subject Areas

General Neuroscience

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10.1016/0304-3940(86)90391-5

Cite this

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title = "Axotomy affects calcium-sensitive potassium conductance in sympathetic neurones",

abstract = "Following peripheral nerve section, the cell body and proximal axon undergo various physiological and morphological changes which may be associated with regrowth. We found that axotomy reduced the amplitude and duration of the afterhyperpolarization (AHP) of the action potential (AP) of bullfrog sympathetic neurones. In addition, the repolarization of the AP was slowed. The Ca2+-channel blocker, Cd2+ (200 μM) produced similar effects in normal cells by blocking the Ca2+-sensitive K+ conductances (gk, Ca) which contribute to the AHP and to AP repolarization. Although Ca2+-channel function was preserved after axotomy, Cd2+ did not affect axotomized neurones. This suggests that axotomy promotes a functional loss of gk, Ca which could reflect a regenerative response of the cell to injury.",

author = "Kelly, {Melanie E.M.} and Tessa Gordon and Jacob Shapiro and Smith, {Peter A.}",

note = "Funding Information: Supported by grants from the Medical Research Council of Canada and the Alberta Heritage Foundation for Medical Research. We thank Dr. R.B. Stein for his comments on the manuscript.",

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AU - Kelly, Melanie E.M.

AU - Gordon, Tessa

AU - Shapiro, Jacob

AU - Smith, Peter A.

N1 - Funding Information: Supported by grants from the Medical Research Council of Canada and the Alberta Heritage Foundation for Medical Research. We thank Dr. R.B. Stein for his comments on the manuscript.

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N2 - Following peripheral nerve section, the cell body and proximal axon undergo various physiological and morphological changes which may be associated with regrowth. We found that axotomy reduced the amplitude and duration of the afterhyperpolarization (AHP) of the action potential (AP) of bullfrog sympathetic neurones. In addition, the repolarization of the AP was slowed. The Ca2+-channel blocker, Cd2+ (200 μM) produced similar effects in normal cells by blocking the Ca2+-sensitive K+ conductances (gk, Ca) which contribute to the AHP and to AP repolarization. Although Ca2+-channel function was preserved after axotomy, Cd2+ did not affect axotomized neurones. This suggests that axotomy promotes a functional loss of gk, Ca which could reflect a regenerative response of the cell to injury.

AB - Following peripheral nerve section, the cell body and proximal axon undergo various physiological and morphological changes which may be associated with regrowth. We found that axotomy reduced the amplitude and duration of the afterhyperpolarization (AHP) of the action potential (AP) of bullfrog sympathetic neurones. In addition, the repolarization of the AP was slowed. The Ca2+-channel blocker, Cd2+ (200 μM) produced similar effects in normal cells by blocking the Ca2+-sensitive K+ conductances (gk, Ca) which contribute to the AHP and to AP repolarization. Although Ca2+-channel function was preserved after axotomy, Cd2+ did not affect axotomized neurones. This suggests that axotomy promotes a functional loss of gk, Ca which could reflect a regenerative response of the cell to injury.

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Axotomy affects calcium-sensitive potassium conductance in sympathetic neurones

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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