TY - JOUR
T1 - BDNF promoter I methylation correlates between post-mortem human peripheral and brain tissues
AU - Stenz, Ludwig
AU - Zewdie, Seblewongel
AU - Laforge-Escarra, Térèse
AU - Prados, Julien
AU - La Harpe, Romano
AU - Dayer, Alexandre
AU - Paoloni-Giacobino, Ariane
AU - Perroud, Nader
AU - Aubry, Jean Michel
N1 - Publisher Copyright:
© 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Several psychiatric disorders have been associated with CpG methylation changes in CG rich promoters of the brain-derived neurotrophic factor (BDNF) mainly by extracting DNA from peripheral blood cells. Whether changes in peripheral DNA methylation can be used as a proxy for brain-specific alterations remains an open question. In this study we aimed to compare DNA methylation levels in BDNF promoter regions in human blood cells, muscle and brain regions using bisulfite-pyrosequencing. We found a significant correlation between the levels of BDNF promoter I methylation measured in quadriceps and vPFC tissues extracted from the same individuals (n=98, Pearson, r=0.48, p=4.5×10-7). In the hippocampus, BDNF promoter I and IV methylation levels were strongly correlated (Pearson, n=37, r=0.74, p=1.4×10-7). We found evidence for sex-dependent effect on BDNF promoter methylation levels in the various tissues and blood samples. Taken together, these data indicate a strong intra-individual correlation between peripheral and brain tissue. They also suggest that sex determines methylation patterns in BDNF promoter region across different types of tissue, including muscle, brain, and blood.
AB - Several psychiatric disorders have been associated with CpG methylation changes in CG rich promoters of the brain-derived neurotrophic factor (BDNF) mainly by extracting DNA from peripheral blood cells. Whether changes in peripheral DNA methylation can be used as a proxy for brain-specific alterations remains an open question. In this study we aimed to compare DNA methylation levels in BDNF promoter regions in human blood cells, muscle and brain regions using bisulfite-pyrosequencing. We found a significant correlation between the levels of BDNF promoter I methylation measured in quadriceps and vPFC tissues extracted from the same individuals (n=98, Pearson, r=0.48, p=4.5×10-7). In the hippocampus, BDNF promoter I and IV methylation levels were strongly correlated (Pearson, n=37, r=0.74, p=1.4×10-7). We found evidence for sex-dependent effect on BDNF promoter methylation levels in the various tissues and blood samples. Taken together, these data indicate a strong intra-individual correlation between peripheral and brain tissue. They also suggest that sex determines methylation patterns in BDNF promoter region across different types of tissue, including muscle, brain, and blood.
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U2 - 10.1016/j.neures.2014.10.003
DO - 10.1016/j.neures.2014.10.003
M3 - Article
C2 - 25450314
AN - SCOPUS:84923883762
SN - 0168-0102
VL - 91
SP - 1
EP - 7
JO - Neuroscience Research
JF - Neuroscience Research
ER -