Canadian association of pathologists-association Canadienne des Pathologistes national standards committee for high complexity testing/Immunohistochemistry guidelines for the preparation, release, and storage of unstained archived diagnostic tissue sections for Immunohistochemistry

Carol C. Cheung, Diponkar Banerjee, Penny J. Barnes, Richard C. Berendt, Jagdish Butany, Sarah Canil, Blaise A. Clarke, Hala El-Zimaity, John Garratt, Laurette Geldenhuys, C. Blake Gilks, Lisa Manning, Michael Mengel, Bayardo Perez-Ordonez, Dragana Pilavdzic, Robert Riddell, Paul E. Swanson, Emina E. Torlakovic

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Objectives: Formalin-fixed, paraffin-embedded unstained archived diagnostic tissue sections are frequently exchanged between clinical laboratories for immunohistochemical staining. The manner in which such sections are prepared represents a type of preanalytical variable that must be taken into account given the growing importance of immunohistochemical assays, especially predictive and prognostic tests, in personalized medicine. Methods: Recommendations were derived from review of the literature and expert consensus of the Canadian Association of Pathologists-Association canadienne des pathologists National Standards Committee for High Complexity Testing/ Immunohistochemistry. Results: Relevant considerations include the type of glass slide on which to mount the unstained sections; the thickness of the tissue sections; the time from slide preparation to testing; the environment, particularly the temperature at which the unstained sections will be maintained prior to testing; the inclusion of on-slide positive control tissue where possible; and whether patient identifier(s) should be included on slide labels.

Original languageEnglish
Pages (from-to)629-633
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume142
Issue number5
DOIs
Publication statusPublished - Nov 2014

Bibliographical note

Publisher Copyright:
© American Society for Clinical Pathology.

ASJC Scopus Subject Areas

  • Pathology and Forensic Medicine

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