TY - JOUR
T1 - Canadian association of pathologists-association Canadienne des Pathologistes national standards committee for high complexity testing/Immunohistochemistry guidelines for the preparation, release, and storage of unstained archived diagnostic tissue sections for Immunohistochemistry
AU - Cheung, Carol C.
AU - Banerjee, Diponkar
AU - Barnes, Penny J.
AU - Berendt, Richard C.
AU - Butany, Jagdish
AU - Canil, Sarah
AU - Clarke, Blaise A.
AU - El-Zimaity, Hala
AU - Garratt, John
AU - Geldenhuys, Laurette
AU - Blake Gilks, C.
AU - Manning, Lisa
AU - Mengel, Michael
AU - Perez-Ordonez, Bayardo
AU - Pilavdzic, Dragana
AU - Riddell, Robert
AU - Swanson, Paul E.
AU - Torlakovic, Emina E.
N1 - Publisher Copyright:
© American Society for Clinical Pathology.
PY - 2014/11
Y1 - 2014/11
N2 - Objectives: Formalin-fixed, paraffin-embedded unstained archived diagnostic tissue sections are frequently exchanged between clinical laboratories for immunohistochemical staining. The manner in which such sections are prepared represents a type of preanalytical variable that must be taken into account given the growing importance of immunohistochemical assays, especially predictive and prognostic tests, in personalized medicine. Methods: Recommendations were derived from review of the literature and expert consensus of the Canadian Association of Pathologists-Association canadienne des pathologists National Standards Committee for High Complexity Testing/ Immunohistochemistry. Results: Relevant considerations include the type of glass slide on which to mount the unstained sections; the thickness of the tissue sections; the time from slide preparation to testing; the environment, particularly the temperature at which the unstained sections will be maintained prior to testing; the inclusion of on-slide positive control tissue where possible; and whether patient identifier(s) should be included on slide labels.
AB - Objectives: Formalin-fixed, paraffin-embedded unstained archived diagnostic tissue sections are frequently exchanged between clinical laboratories for immunohistochemical staining. The manner in which such sections are prepared represents a type of preanalytical variable that must be taken into account given the growing importance of immunohistochemical assays, especially predictive and prognostic tests, in personalized medicine. Methods: Recommendations were derived from review of the literature and expert consensus of the Canadian Association of Pathologists-Association canadienne des pathologists National Standards Committee for High Complexity Testing/ Immunohistochemistry. Results: Relevant considerations include the type of glass slide on which to mount the unstained sections; the thickness of the tissue sections; the time from slide preparation to testing; the environment, particularly the temperature at which the unstained sections will be maintained prior to testing; the inclusion of on-slide positive control tissue where possible; and whether patient identifier(s) should be included on slide labels.
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U2 - 10.1309/AJCP77GXYVEQXMXT
DO - 10.1309/AJCP77GXYVEQXMXT
M3 - Article
C2 - 25319977
AN - SCOPUS:84917735896
SN - 0002-9173
VL - 142
SP - 629
EP - 633
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 5
ER -