Carbon dioxide mediated glycolysis II

Danuta T. Zborowska-Sluis; Gerald A. Klassen

doi:10.1016/0034-5687(73)90075-3

Carbon dioxide mediated glycolysis II

Danuta T. Zborowska-Sluis, Gerald A. Klassen

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Prolonged incubation (5 hr) of canine erythrocyte at pH 7.2, or pH 7.7 and P_CO₂ 3.5 or 35 were used to determine the effect of CO₂ on glycolysis. The following was observed: 1) an inverse relationship exists between lactate production and CO₂. 2) the inhibitory effect of H⁺ ion on glucose uptake is decreased by prolonged incubation (5 hr compared to 1 hr). 3) carbon dioxide becomes inhibitory for glucose uptake following prolonged incubation. 4) increasing external bicarbonate decreases erythrocyte 2-3 DPG. 5) iodoacetate inhibition of glycolysis reverses the inverse relationship of lactate (pyruvate) to CO₂ suggesting that the CO₂ sensitive enzyme lies proximal to 1-3 diphosphoglycerate.

Original language	English
Pages (from-to)	162-175
Number of pages	14
Journal	Respiration Physiology
Volume	19
Issue number	2
DOIs	https://doi.org/10.1016/0034-5687(73)90075-3
Publication status	Published - Nov 1973
Externally published	Yes

Bibliographical note

Funding Information:
The technical assistance of Mrs. Edie Summers and Mrs. Heather Irving, and the secretarial expertise of Miss Bridget Byrne was appreciated. This work was supported by grants from the Medical Research Council of Canada and the Muscular Dystrophy Association of Canada.

ASJC Scopus Subject Areas

Physiology
Pulmonary and Respiratory Medicine

PubMed: MeSH publication types

Journal Article

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10.1016/0034-5687(73)90075-3

Cite this

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title = "Carbon dioxide mediated glycolysis II",

abstract = "Prolonged incubation (5 hr) of canine erythrocyte at pH 7.2, or pH 7.7 and PCO2 3.5 or 35 were used to determine the effect of CO2 on glycolysis. The following was observed: 1) an inverse relationship exists between lactate production and CO2. 2) the inhibitory effect of H+ ion on glucose uptake is decreased by prolonged incubation (5 hr compared to 1 hr). 3) carbon dioxide becomes inhibitory for glucose uptake following prolonged incubation. 4) increasing external bicarbonate decreases erythrocyte 2-3 DPG. 5) iodoacetate inhibition of glycolysis reverses the inverse relationship of lactate (pyruvate) to CO2 suggesting that the CO2 sensitive enzyme lies proximal to 1-3 diphosphoglycerate.",

author = "Zborowska-Sluis, {Danuta T.} and Klassen, {Gerald A.}",

note = "Funding Information: The technical assistance of Mrs. Edie Summers and Mrs. Heather Irving, and the secretarial expertise of Miss Bridget Byrne was appreciated. This work was supported by grants from the Medical Research Council of Canada and the Muscular Dystrophy Association of Canada.",

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doi = "10.1016/0034-5687(73)90075-3",

language = "English",

volume = "19",

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T1 - Carbon dioxide mediated glycolysis II

AU - Zborowska-Sluis, Danuta T.

AU - Klassen, Gerald A.

N1 - Funding Information: The technical assistance of Mrs. Edie Summers and Mrs. Heather Irving, and the secretarial expertise of Miss Bridget Byrne was appreciated. This work was supported by grants from the Medical Research Council of Canada and the Muscular Dystrophy Association of Canada.

PY - 1973/11

Y1 - 1973/11

N2 - Prolonged incubation (5 hr) of canine erythrocyte at pH 7.2, or pH 7.7 and PCO2 3.5 or 35 were used to determine the effect of CO2 on glycolysis. The following was observed: 1) an inverse relationship exists between lactate production and CO2. 2) the inhibitory effect of H+ ion on glucose uptake is decreased by prolonged incubation (5 hr compared to 1 hr). 3) carbon dioxide becomes inhibitory for glucose uptake following prolonged incubation. 4) increasing external bicarbonate decreases erythrocyte 2-3 DPG. 5) iodoacetate inhibition of glycolysis reverses the inverse relationship of lactate (pyruvate) to CO2 suggesting that the CO2 sensitive enzyme lies proximal to 1-3 diphosphoglycerate.

AB - Prolonged incubation (5 hr) of canine erythrocyte at pH 7.2, or pH 7.7 and PCO2 3.5 or 35 were used to determine the effect of CO2 on glycolysis. The following was observed: 1) an inverse relationship exists between lactate production and CO2. 2) the inhibitory effect of H+ ion on glucose uptake is decreased by prolonged incubation (5 hr compared to 1 hr). 3) carbon dioxide becomes inhibitory for glucose uptake following prolonged incubation. 4) increasing external bicarbonate decreases erythrocyte 2-3 DPG. 5) iodoacetate inhibition of glycolysis reverses the inverse relationship of lactate (pyruvate) to CO2 suggesting that the CO2 sensitive enzyme lies proximal to 1-3 diphosphoglycerate.

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Carbon dioxide mediated glycolysis II

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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