Change in prostaglandin signaling during sickness syndrome hyperalgesia after ovariectomy in female rats

I. K. Maba; J. V. Cruz; A. R. Zampronio

doi:10.1016/j.bbr.2021.113368

Change in prostaglandin signaling during sickness syndrome hyperalgesia after ovariectomy in female rats

I. K. Maba, J. V. Cruz, A. R. Zampronio

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2 Citations (Scopus)

Abstract

The present study investigated hyperalgesia during sickness syndrome in female rats. Hyperalgesia was induced by an intraperitoneal injection of lipopolysaccharide (LPS) or an intracerebroventricular injection of prostaglandin E₂ (PGE₂). No differences were found in basal mechanical and thermal thresholds or in LPS-induced hyperalgesia in sham-operated animals in the diestrus or proestrus phase or in ovariectomized (OVX) animals. However, higher levels of PGE₂ where found in the cerebrospinal fluid of OVX animals compared to sham-operated females. Intracerebroventricular injection of PGE₂ produced rapid mechanical hyperalgesia in sham-operated rats while these responses were observed at later times in OVX animals. The protein kinase A (PKA) inhibitor H-89 reduced mechanical PGE₂-induced hyperalgesia in OVX female rats, whereas no effect was observed in sham-operated animals. In contrast, the exchange protein activated by cyclic adenosine monophosphate (cAMP; Epac) inhibitor ESI-09 reduced mechanical PGE₂-induced hyperalgesia, whereas no effect was observed in OVX animals. PGE₂ also induced thermal hyperalgesia in sham-operated and OVX female rats and a similar effect of ESI-09 was observed. These results suggest that PGE₂-induced hyperalgesia that is observed during sickness syndrome has different signaling mechanisms in cycling and OVX female rats involving the activation of the cAMP-Epac or cAMP-PKA pathways, respectively.

Original language	English
Article number	113368
Journal	Behavioural Brain Research
Volume	410
DOIs	https://doi.org/10.1016/j.bbr.2021.113368
Publication status	Published - Jul 23 2021
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant # 457938/2014-5 ). IKM and JVC are recipients of fellowships from CNPq and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) .

Funding Information:
This study was supported by Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq, grant # 457938/2014-5). IKM and JVC are recipients of fellowships from CNPq and Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES).

Publisher Copyright:
© 2021

ASJC Scopus Subject Areas

Behavioral Neuroscience

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/j.bbr.2021.113368

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title = "Change in prostaglandin signaling during sickness syndrome hyperalgesia after ovariectomy in female rats",

abstract = "The present study investigated hyperalgesia during sickness syndrome in female rats. Hyperalgesia was induced by an intraperitoneal injection of lipopolysaccharide (LPS) or an intracerebroventricular injection of prostaglandin E2 (PGE2). No differences were found in basal mechanical and thermal thresholds or in LPS-induced hyperalgesia in sham-operated animals in the diestrus or proestrus phase or in ovariectomized (OVX) animals. However, higher levels of PGE2 where found in the cerebrospinal fluid of OVX animals compared to sham-operated females. Intracerebroventricular injection of PGE2 produced rapid mechanical hyperalgesia in sham-operated rats while these responses were observed at later times in OVX animals. The protein kinase A (PKA) inhibitor H-89 reduced mechanical PGE2-induced hyperalgesia in OVX female rats, whereas no effect was observed in sham-operated animals. In contrast, the exchange protein activated by cyclic adenosine monophosphate (cAMP; Epac) inhibitor ESI-09 reduced mechanical PGE2-induced hyperalgesia, whereas no effect was observed in OVX animals. PGE2 also induced thermal hyperalgesia in sham-operated and OVX female rats and a similar effect of ESI-09 was observed. These results suggest that PGE2-induced hyperalgesia that is observed during sickness syndrome has different signaling mechanisms in cycling and OVX female rats involving the activation of the cAMP-Epac or cAMP-PKA pathways, respectively.",

author = "Maba, {I. K.} and Cruz, {J. V.} and Zampronio, {A. R.}",

note = "Funding Information: This study was supported by Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq, grant # 457938/2014-5 ). IKM and JVC are recipients of fellowships from CNPq and Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior (CAPES) . Funding Information: This study was supported by Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq, grant # 457938/2014-5). IKM and JVC are recipients of fellowships from CNPq and Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES). Publisher Copyright: {\textcopyright} 2021",

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Change in prostaglandin signaling during sickness syndrome hyperalgesia after ovariectomy in female rats

Abstract

Bibliographical note

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PubMed: MeSH publication types

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