Abstract
Vitellogenin (VTG) is a protein produced by the liver of oviparous animals. It is being used as a biomarker for exposure to endocrine disruptors in many species. Rainbow trout Vtg has recently been sequenced by the conventional cDNA nucleotide approach. We focused on protein characterization of the intact protein and its derived tryptic and cyanogen bromide peptides by matrix-assisted laser desorption/ionisation and electrospray ionisation mass spectrometry. The molecular mass of the intact protein was found to be 183127 Da. A large number of unidentified peptide ions encourage further structural analysis to propose possible sequence variants and post-translational modifications.
Original language | English |
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Pages (from-to) | 509-524 |
Number of pages | 16 |
Journal | European Journal of Mass Spectrometry |
Volume | 9 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2003 |
Externally published | Yes |
ASJC Scopus Subject Areas
- Atomic and Molecular Physics, and Optics
- Spectroscopy