Characterization of a novel adhesion function blocking monoclonal antibody to rat/mouse P-selectin

U. M. Walter; L. Aver; B. A. Wolitzky; D. Wagner; R. O. Hynes; A. M. Manning; A. C. Issekutz

Characterization of a novel adhesion function blocking monoclonal antibody to rat/mouse P-selectin

U. M. Walter, L. Aver, B. A. Wolitzky, D. Wagner, R. O. Hynes, A. M. Manning, A. C. Issekutz

Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

In a P-selectin knockout mouse we generated an anti-rat/mouse P-selectin monoclonal antibody (mAb), designated RMP-1, by immunization with activated rat platelets. This IgG2a mAb immunoprecipitates a 140 kDa protein under reducing conditions from rat platelet lysate, and also reacts in Western blotting under non-reducing conditions. RMP-1 reacts with fixed and unfixed thrombin activated, but not unactivated rat platelets as well as with recombinant rat- and mouse P-selectin protein as assessed by ELISA. Flow cytometry analysis revealed binding of RMP-1 to activated rat platelets. MAb RMP-1 inhibited adhesion of HL-60 cells to immobilized mouse P-selectin by 91% and to activated rat platelets by 80% under static conditions. This novel adhesion blocking antibody should be useful for studying P-selectin function jn vitro and in vivo in rat and mouse inflammation models.

Original language	English
Pages (from-to)	A1199
Journal	FASEB Journal
Volume	10
Issue number	6
Publication status	Published - 1996

ASJC Scopus Subject Areas

Biotechnology
Biochemistry
Molecular Biology
Genetics

Cite this

Walter, U. M., Aver, L., Wolitzky, B. A., Wagner, D., Hynes, R. O., Manning, A. M., & Issekutz, A. C. (1996). Characterization of a novel adhesion function blocking monoclonal antibody to rat/mouse P-selectin. FASEB Journal, 10(6), A1199.

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abstract = "In a P-selectin knockout mouse we generated an anti-rat/mouse P-selectin monoclonal antibody (mAb), designated RMP-1, by immunization with activated rat platelets. This IgG2a mAb immunoprecipitates a 140 kDa protein under reducing conditions from rat platelet lysate, and also reacts in Western blotting under non-reducing conditions. RMP-1 reacts with fixed and unfixed thrombin activated, but not unactivated rat platelets as well as with recombinant rat- and mouse P-selectin protein as assessed by ELISA. Flow cytometry analysis revealed binding of RMP-1 to activated rat platelets. MAb RMP-1 inhibited adhesion of HL-60 cells to immobilized mouse P-selectin by 91% and to activated rat platelets by 80% under static conditions. This novel adhesion blocking antibody should be useful for studying P-selectin function jn vitro and in vivo in rat and mouse inflammation models.",

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AU - Walter, U. M.

AU - Aver, L.

AU - Wolitzky, B. A.

AU - Wagner, D.

AU - Hynes, R. O.

AU - Manning, A. M.

AU - Issekutz, A. C.

PY - 1996

Y1 - 1996

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AB - In a P-selectin knockout mouse we generated an anti-rat/mouse P-selectin monoclonal antibody (mAb), designated RMP-1, by immunization with activated rat platelets. This IgG2a mAb immunoprecipitates a 140 kDa protein under reducing conditions from rat platelet lysate, and also reacts in Western blotting under non-reducing conditions. RMP-1 reacts with fixed and unfixed thrombin activated, but not unactivated rat platelets as well as with recombinant rat- and mouse P-selectin protein as assessed by ELISA. Flow cytometry analysis revealed binding of RMP-1 to activated rat platelets. MAb RMP-1 inhibited adhesion of HL-60 cells to immobilized mouse P-selectin by 91% and to activated rat platelets by 80% under static conditions. This novel adhesion blocking antibody should be useful for studying P-selectin function jn vitro and in vivo in rat and mouse inflammation models.

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Characterization of a novel adhesion function blocking monoclonal antibody to rat/mouse P-selectin

Abstract

ASJC Scopus Subject Areas

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