Characterization of an epithelioid cell line derived from rat small intestine: Demonstration of cytokeratin filaments

J. Blay; K. D. Brown

doi:10.1016/0309-1651(84)90054-7

Characterization of an epithelioid cell line derived from rat small intestine: Demonstration of cytokeratin filaments

J. Blay, K. D. Brown

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Abstract

A cell line (RIE-1) of epithelioid morphology was established from rat small intestine. RIE-1 cells possess the normal rat diploid number of chromosomes (42) up to at least passage 20. Both early (< 10) and late (>20) passage cells are non-tumourigenic and do not form colonies in soft agarose. RIE-1 cells bind two epithelial-specific monoclonal antibodies. In addition, the cells exhibit a network of cytoskeletal filaments which are labelled by an antiserum to keratin. These findings provide strong evidence for an epithelial origin of the RIE-1 cell line.

Original language	English
Pages (from-to)	551-560
Number of pages	10
Journal	Cell Biology International Reports
Volume	8
Issue number	7
DOIs	https://doi.org/10.1016/0309-1651(84)90054-7
Publication status	Published - Jul 1984
Externally published	Yes

ASJC Scopus Subject Areas

Cell Biology

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abstract = "A cell line (RIE-1) of epithelioid morphology was established from rat small intestine. RIE-1 cells possess the normal rat diploid number of chromosomes (42) up to at least passage 20. Both early (< 10) and late (>20) passage cells are non-tumourigenic and do not form colonies in soft agarose. RIE-1 cells bind two epithelial-specific monoclonal antibodies. In addition, the cells exhibit a network of cytoskeletal filaments which are labelled by an antiserum to keratin. These findings provide strong evidence for an epithelial origin of the RIE-1 cell line.",

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AB - A cell line (RIE-1) of epithelioid morphology was established from rat small intestine. RIE-1 cells possess the normal rat diploid number of chromosomes (42) up to at least passage 20. Both early (< 10) and late (>20) passage cells are non-tumourigenic and do not form colonies in soft agarose. RIE-1 cells bind two epithelial-specific monoclonal antibodies. In addition, the cells exhibit a network of cytoskeletal filaments which are labelled by an antiserum to keratin. These findings provide strong evidence for an epithelial origin of the RIE-1 cell line.

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Characterization of an epithelioid cell line derived from rat small intestine: Demonstration of cytokeratin filaments

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