Abstract
Nineteen hybridoma cell lines derived from the spleen cells of a mouse immunized with reovirus serotype 3 (strain Dearing) were cloned and the IgGs secreted by them characterized. Four of the IgGs were directed against polypeptide μNS and two against polypeptide σNS, the two nonstructural reovirus-specified polypeptides. Two IgGs were directed against polypeptide λ2 and two against polypeptide σ2, both components of the reovirus inner capsid shell. Four IgGs were directed against polypeptide σ1, the most type specific of all reovirus-specified polypeptides, three against polypeptide μl/gmlC and one against polypeptide σ3, all of them components of the reovirus outer capsid shell. One IgG was directed against a complex composed of polypeptides μl/gmlC and σ3, but failed to react with either of these polypeptides alone. Thus the 19 hybridoma cell lines secreted IgGs with specificities for 7 of the 10 reovirus-specified proteins. The ability of all these IgGs to precipitate the proteins specified by the two heterologous reovirus serotypes, serotypes 1 and 2, was also measured.
| Original language | English |
|---|---|
| Pages (from-to) | 134-146 |
| Number of pages | 13 |
| Journal | Virology |
| Volume | 108 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 15 1981 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by Research Grants ROl AI 08909 and ROl AI 10132, and by Research Training Grant T32 CA 09111 from the NIH. P. W. K. Lee was the recipient of a Medical Research Council of Canada Fellowship. We would like to acknowledge with gratitude the helpful discussions with Scott Anderson, a graduate student in this department who introduced us to hybridoma technology, and the expert technical assistance of Sherry Montgomery.
ASJC Scopus Subject Areas
- Virology