Combining High-Throughput Sequencing of sedaDNA and Traditional Paleolimnological Techniques to Infer Historical Trends in Cyanobacterial Communities

Timothy J. Tse; Lorne E. Doig; Song Tang; Xiaohui Zhang; Weimin Sun; Steve B. Wiseman; Cindy Xin Feng; Hongling Liu; John P. Giesy; Markus Hecker; Paul D. Jones

doi:10.1021/acs.est.7b06386

Combining High-Throughput Sequencing of sedaDNA and Traditional Paleolimnological Techniques to Infer Historical Trends in Cyanobacterial Communities

Timothy J. Tse, Lorne E. Doig, Song Tang, Xiaohui Zhang, Weimin Sun, Steve B. Wiseman, Cindy Xin Feng, Hongling Liu, John P. Giesy, Markus Hecker, Paul D. Jones

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Freshwaters worldwide are under increasing pressure from anthropogenic activities and changing climate. Unfortunately, many inland waters lack sufficient long-term monitoring to assess environmental trends. Analysis of sedimentary ancient DNA (sedaDNA) is emerging as a means to reconstruct the past occurrence of microbial communities of inland waters. The purpose of this study was to assess a combination of high-throughput sequencing (16S rRNA) of sedaDNA and traditional paleolimnological analyses to explore multidecadal relationships among cyanobacterial community composition, the potential for cyanotoxin production, and paleoenvironmental proxies. DNA was extracted from two sediment cores collected from a northern Canadian Great Plains reservoir. Diversity indices illustrated significant community-level changes since reservoir formation. Furthermore, higher relative abundances in more recent years were observed for potentially toxic cyanobacterial genera including Dolichospermum. Correlation-based network analysis revealed this trend significantly and positively correlated to abundances of the microcystin synthetase gene (mcyA) and other paleoproxies (nutrients, pigments, stanols, sterols, and certain diatom species), demonstrating synchrony between molecular and more standard proxies. These findings demonstrate a novel approach to infer long-term dynamics of cyanobacterial diversity in inland waters and highlight the power of high-throughput sequencing to reconstruct trends in environmental quality and inform lake and reservoir management and monitoring program design.

Original language	English
Pages (from-to)	6842-6853
Number of pages	12
Journal	Environmental Science and Technology
Volume	52
Issue number	12
DOIs	https://doi.org/10.1021/acs.est.7b06386
Publication status	Published - Jun 19 2018
Externally published	Yes

Bibliographical note

Funding Information:
Funding for this project was provided by the Global Institute of Water Security in University of Saskatchewan, Saskatchewan, Canada through the Canada Excellence Research Chair in Water Security and the Natural Sciences and Engineering Research Council of Canada. J.P.G. and M.H. were supported by the Canada Research Chair program. The research was also supported by a Discovery Grant from the Natural Science and Engineering Research Council of Canada to J. (Project # 326415-07) and a Start-up Funding from NIEH, China CDC to S.T. W.S. was funded through the high-level leading talent introduction program of Guangdong Academy of Science in China.

Publisher Copyright:
© 2018 American Chemical Society.

ASJC Scopus Subject Areas

General Chemistry
Environmental Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.est.7b06386

Cite this

Tse, T. J., Doig, L. E., Tang, S., Zhang, X., Sun, W., Wiseman, S. B., Feng, C. X., Liu, H., Giesy, J. P., Hecker, M., & Jones, P. D. (2018). Combining High-Throughput Sequencing of sedaDNA and Traditional Paleolimnological Techniques to Infer Historical Trends in Cyanobacterial Communities. Environmental Science and Technology, 52(12), 6842-6853. https://doi.org/10.1021/acs.est.7b06386

Tse, TJ, Doig, LE, Tang, S, Zhang, X, Sun, W, Wiseman, SB, Feng, CX, Liu, H, Giesy, JP, Hecker, M & Jones, PD 2018, 'Combining High-Throughput Sequencing of sedaDNA and Traditional Paleolimnological Techniques to Infer Historical Trends in Cyanobacterial Communities', Environmental Science and Technology, vol. 52, no. 12, pp. 6842-6853. https://doi.org/10.1021/acs.est.7b06386

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title = "Combining High-Throughput Sequencing of sedaDNA and Traditional Paleolimnological Techniques to Infer Historical Trends in Cyanobacterial Communities",

abstract = "Freshwaters worldwide are under increasing pressure from anthropogenic activities and changing climate. Unfortunately, many inland waters lack sufficient long-term monitoring to assess environmental trends. Analysis of sedimentary ancient DNA (sedaDNA) is emerging as a means to reconstruct the past occurrence of microbial communities of inland waters. The purpose of this study was to assess a combination of high-throughput sequencing (16S rRNA) of sedaDNA and traditional paleolimnological analyses to explore multidecadal relationships among cyanobacterial community composition, the potential for cyanotoxin production, and paleoenvironmental proxies. DNA was extracted from two sediment cores collected from a northern Canadian Great Plains reservoir. Diversity indices illustrated significant community-level changes since reservoir formation. Furthermore, higher relative abundances in more recent years were observed for potentially toxic cyanobacterial genera including Dolichospermum. Correlation-based network analysis revealed this trend significantly and positively correlated to abundances of the microcystin synthetase gene (mcyA) and other paleoproxies (nutrients, pigments, stanols, sterols, and certain diatom species), demonstrating synchrony between molecular and more standard proxies. These findings demonstrate a novel approach to infer long-term dynamics of cyanobacterial diversity in inland waters and highlight the power of high-throughput sequencing to reconstruct trends in environmental quality and inform lake and reservoir management and monitoring program design.",

author = "Tse, {Timothy J.} and Doig, {Lorne E.} and Song Tang and Xiaohui Zhang and Weimin Sun and Wiseman, {Steve B.} and Feng, {Cindy Xin} and Hongling Liu and Giesy, {John P.} and Markus Hecker and Jones, {Paul D.}",

note = "Funding Information: Funding for this project was provided by the Global Institute of Water Security in University of Saskatchewan, Saskatchewan, Canada through the Canada Excellence Research Chair in Water Security and the Natural Sciences and Engineering Research Council of Canada. J.P.G. and M.H. were supported by the Canada Research Chair program. The research was also supported by a Discovery Grant from the Natural Science and Engineering Research Council of Canada to J. (Project # 326415-07) and a Start-up Funding from NIEH, China CDC to S.T. W.S. was funded through the high-level leading talent introduction program of Guangdong Academy of Science in China. Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

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AU - Tang, Song

AU - Zhang, Xiaohui

AU - Sun, Weimin

AU - Wiseman, Steve B.

AU - Feng, Cindy Xin

AU - Liu, Hongling

AU - Giesy, John P.

AU - Hecker, Markus

AU - Jones, Paul D.

N1 - Funding Information: Funding for this project was provided by the Global Institute of Water Security in University of Saskatchewan, Saskatchewan, Canada through the Canada Excellence Research Chair in Water Security and the Natural Sciences and Engineering Research Council of Canada. J.P.G. and M.H. were supported by the Canada Research Chair program. The research was also supported by a Discovery Grant from the Natural Science and Engineering Research Council of Canada to J. (Project # 326415-07) and a Start-up Funding from NIEH, China CDC to S.T. W.S. was funded through the high-level leading talent introduction program of Guangdong Academy of Science in China. Publisher Copyright: © 2018 American Chemical Society.

PY - 2018/6/19

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N2 - Freshwaters worldwide are under increasing pressure from anthropogenic activities and changing climate. Unfortunately, many inland waters lack sufficient long-term monitoring to assess environmental trends. Analysis of sedimentary ancient DNA (sedaDNA) is emerging as a means to reconstruct the past occurrence of microbial communities of inland waters. The purpose of this study was to assess a combination of high-throughput sequencing (16S rRNA) of sedaDNA and traditional paleolimnological analyses to explore multidecadal relationships among cyanobacterial community composition, the potential for cyanotoxin production, and paleoenvironmental proxies. DNA was extracted from two sediment cores collected from a northern Canadian Great Plains reservoir. Diversity indices illustrated significant community-level changes since reservoir formation. Furthermore, higher relative abundances in more recent years were observed for potentially toxic cyanobacterial genera including Dolichospermum. Correlation-based network analysis revealed this trend significantly and positively correlated to abundances of the microcystin synthetase gene (mcyA) and other paleoproxies (nutrients, pigments, stanols, sterols, and certain diatom species), demonstrating synchrony between molecular and more standard proxies. These findings demonstrate a novel approach to infer long-term dynamics of cyanobacterial diversity in inland waters and highlight the power of high-throughput sequencing to reconstruct trends in environmental quality and inform lake and reservoir management and monitoring program design.

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Combining High-Throughput Sequencing of sedaDNA and Traditional Paleolimnological Techniques to Infer Historical Trends in Cyanobacterial Communities

Abstract

Bibliographical note

ASJC Scopus Subject Areas

UN SDGs

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