Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein–Protein Interactions

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

G protein-coupled receptors (GPCRs) are the target for many drugs. Evidence continues to accumulate demonstrating that multiple receptors form homo- and heteromeric complexes, which in turn dynamically couple with G proteins, and other interacting proteins. Here, we describe a method to simultaneously determine the identity of up to four distinct constituents of GPCR complexes using a combination of sequential bioluminescence resonance energy transfer 2—fluorescence resonance energy transfer (SRET2) with bimolecular fluorescence complementation (BiFC). The method is amenable to moderate throughput screening of changes in response to ligands and time-course analysis of protein–protein oligomerization.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-215
Number of pages17
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1947
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

  • Molecular Biology
  • Genetics

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