Comparison of the biochemical composition of normal epidermal mucus and extruded slime of hagfish (Myxine glutinosa L.)

S. Subramanian; N. W. Ross; S. L. MacKinnon

doi:10.1016/j.fsi.2008.08.012

Comparison of the biochemical composition of normal epidermal mucus and extruded slime of hagfish (Myxine glutinosa L.)

S. Subramanian, N. W. Ross, S. L. MacKinnon

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Hagfish (Myxine glutinosa) secrete normal epidermal mucus and extruded slime. The epidermal mucus is produced continuously to prevent pathogen adherence while the extruded slime is observed predominantly during feeding, provocation or stress. To date little is known about the involvement of extruded slime in the physiological functions of hagfish. In this preliminary study, innate immune enzymes and the protein composition of hagfish normal epidermal mucus and extruded slime were analysed and compared. The lysozyme specific activity of hagfish was observed approximately two-fold higher in extruded slime than that of epidermal mucus. The extruded slime had approximately 3.5-5.0 fold increased levels of alkaline phosphatase, cathepsin B and proteases in comparison to epidermal mucus. Protease characterization using specific inhibitors showed that the extruded slime had higher levels of serine trypsin-like proteases compared to metalloproteases whereas epidermal mucus showed equal proportion of both serine and metalloproteases. SDS-PAGE analysis showed high levels of three proteins with molecular masses in the range of 13-16 kDa in the extruded slime. The LC/MS/MS analysis of protein bands 1, 2 and 3 showed closest matches to hemoglobulin-3, histone H3 and H2B proteins, respectively. The observation of elevated levels of innate immune parameters in the extruded slime suggested that the extruded slime has a significant role in innate immunity of hagfish against infectious pathogens. Crown

Original language	English
Pages (from-to)	625-632
Number of pages	8
Journal	Fish and Shellfish Immunology
Volume	25
Issue number	5
DOIs	https://doi.org/10.1016/j.fsi.2008.08.012
Publication status	Published - Nov 2008
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Mr. Eric MacKinnon (Cape Sable Island, NS) for providing hagfishes and Ron Melanson for help with sampling. Elden Rowland and Roger Ebanks for assistance with the mass spectrometric analysis. This research was funded by National Research Council of Canada and the Ford Foundation International Fellowship Program.

ASJC Scopus Subject Areas

Environmental Chemistry
Immunology
Aquatic Science
Immunology and Microbiology (miscellaneous)

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10.1016/j.fsi.2008.08.012

Cite this

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title = "Comparison of the biochemical composition of normal epidermal mucus and extruded slime of hagfish (Myxine glutinosa L.)",

abstract = "Hagfish (Myxine glutinosa) secrete normal epidermal mucus and extruded slime. The epidermal mucus is produced continuously to prevent pathogen adherence while the extruded slime is observed predominantly during feeding, provocation or stress. To date little is known about the involvement of extruded slime in the physiological functions of hagfish. In this preliminary study, innate immune enzymes and the protein composition of hagfish normal epidermal mucus and extruded slime were analysed and compared. The lysozyme specific activity of hagfish was observed approximately two-fold higher in extruded slime than that of epidermal mucus. The extruded slime had approximately 3.5-5.0 fold increased levels of alkaline phosphatase, cathepsin B and proteases in comparison to epidermal mucus. Protease characterization using specific inhibitors showed that the extruded slime had higher levels of serine trypsin-like proteases compared to metalloproteases whereas epidermal mucus showed equal proportion of both serine and metalloproteases. SDS-PAGE analysis showed high levels of three proteins with molecular masses in the range of 13-16 kDa in the extruded slime. The LC/MS/MS analysis of protein bands 1, 2 and 3 showed closest matches to hemoglobulin-3, histone H3 and H2B proteins, respectively. The observation of elevated levels of innate immune parameters in the extruded slime suggested that the extruded slime has a significant role in innate immunity of hagfish against infectious pathogens. Crown",

author = "S. Subramanian and Ross, {N. W.} and MacKinnon, {S. L.}",

note = "Funding Information: The authors would like to thank Mr. Eric MacKinnon (Cape Sable Island, NS) for providing hagfishes and Ron Melanson for help with sampling. Elden Rowland and Roger Ebanks for assistance with the mass spectrometric analysis. This research was funded by National Research Council of Canada and the Ford Foundation International Fellowship Program.",

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Comparison of the biochemical composition of normal epidermal mucus and extruded slime of hagfish (Myxine glutinosa L.)

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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