Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental antarctica

Peter Hirsch; Claudia A. Gallikowski; Jörg Siebert; Klaus Peissl; Reiner Kroppenstedt; Peter Schumann; Erko Stackebrandt; Robert Anderson

doi:10.1078/0723202042370008

Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental antarctica

Peter Hirsch, Claudia A. Gallikowski, Jörg Siebert, Klaus Peissl, Reiner Kroppenstedt, Peter Schumann, Erko Stackebrandt, Robert Anderson

Medicine

Research output: Contribution to journal › Article › peer-review

128 Citations (Scopus)

Abstract

Six Gram-positive, non-motile, UV- and draught-tolerant bacteria were isolated from antarctic soil and rock samples. The pink to orange cocci grew well on oligotrophic medium PYGV (pH 7.5) at 9-18°C. They tolerated 0-10 % NaCl, were aerobic to facultatively anaerobic and contained ornithine in their cell wall (type A3β, Orn-Gly₂). The lipid profiles of four strains were found to be typical for those of D. radiodurans. Major fatty acids were 16:1cis9, 15:1cis9, 17:1cis9 and i17:1cis9, the respiratory quinone of three strains was MK-8. Comparative 16S rDNA gene sequencing revealed phylogenetic relationships to the Deinococcus clade, especially to D. radiopugnans. The levels of 16S rRNA gene sequence similarity and DNA-DNA hybridisation data showed the six isolates represented new taxa. Phenotypic properties supported the description of three new species which were different from the eight known Deinococcus species and particularly from D. radiopugnans. Soil isolate AA-692^T (DSM 12807^T) is the type strain of Deinococcus frigens sp. nov., with AA-752 (DSM 15993) and AA-829 (DSM 15994) as additional strains from soil. The endolithic isolate AA-1444^T, Deinococcus saxicola sp. nov., (DSM 15974^T) came from antarctic sandstone, and Deinococcus marmoris sp. nov. (isolate AA-63^T [DSM 12784^T]) as well as AA-69 (DSM 15951) were isolated from antarctic marble.

Original language	English
Pages (from-to)	636-645
Number of pages	10
Journal	Systematic and Applied Microbiology
Volume	27
Issue number	6
DOIs	https://doi.org/10.1078/0723202042370008
Publication status	Published - Nov 2004

Bibliographical note

Funding Information:
Skillful technical assistance of B. Hoffmann, A. Gräter and M. Beese is gratefully acknowledged. We thank H. G. Trüper for his taxonomic and nomenclatural advice. We are especially grateful to the US National Science Foundation (Polar Program) and E. Imre Friedmann (Tallahassee, Florida) for generous support during three antarctic expeditions on his personal grant (DPP83-14180) and for his hospitality in his laboratory. The Deutsche Forschungsgemeinschaft supported P. H. (grants Hi 68/13/1-5).

ASJC Scopus Subject Areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Applied Microbiology and Biotechnology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

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10.1078/0723202042370008

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Hirsch, P., Gallikowski, C. A., Siebert, J., Peissl, K., Kroppenstedt, R., Schumann, P., Stackebrandt, E., & Anderson, R. (2004). Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental antarctica. Systematic and Applied Microbiology, 27(6), 636-645. https://doi.org/10.1078/0723202042370008

Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental antarctica. / Hirsch, Peter; Gallikowski, Claudia A.; Siebert, Jörg et al.
In: Systematic and Applied Microbiology, Vol. 27, No. 6, 11.2004, p. 636-645.

Research output: Contribution to journal › Article › peer-review

Hirsch, P, Gallikowski, CA, Siebert, J, Peissl, K, Kroppenstedt, R, Schumann, P, Stackebrandt, E & Anderson, R 2004, 'Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental antarctica', Systematic and Applied Microbiology, vol. 27, no. 6, pp. 636-645. https://doi.org/10.1078/0723202042370008

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abstract = "Six Gram-positive, non-motile, UV- and draught-tolerant bacteria were isolated from antarctic soil and rock samples. The pink to orange cocci grew well on oligotrophic medium PYGV (pH 7.5) at 9-18°C. They tolerated 0-10 % NaCl, were aerobic to facultatively anaerobic and contained ornithine in their cell wall (type A3β, Orn-Gly2). The lipid profiles of four strains were found to be typical for those of D. radiodurans. Major fatty acids were 16:1cis9, 15:1cis9, 17:1cis9 and i17:1cis9, the respiratory quinone of three strains was MK-8. Comparative 16S rDNA gene sequencing revealed phylogenetic relationships to the Deinococcus clade, especially to D. radiopugnans. The levels of 16S rRNA gene sequence similarity and DNA-DNA hybridisation data showed the six isolates represented new taxa. Phenotypic properties supported the description of three new species which were different from the eight known Deinococcus species and particularly from D. radiopugnans. Soil isolate AA-692T (DSM 12807T) is the type strain of Deinococcus frigens sp. nov., with AA-752 (DSM 15993) and AA-829 (DSM 15994) as additional strains from soil. The endolithic isolate AA-1444T, Deinococcus saxicola sp. nov., (DSM 15974T) came from antarctic sandstone, and Deinococcus marmoris sp. nov. (isolate AA-63T [DSM 12784T]) as well as AA-69 (DSM 15951) were isolated from antarctic marble.",

author = "Peter Hirsch and Gallikowski, {Claudia A.} and J{\"o}rg Siebert and Klaus Peissl and Reiner Kroppenstedt and Peter Schumann and Erko Stackebrandt and Robert Anderson",

note = "Funding Information: Skillful technical assistance of B. Hoffmann, A. Gr{\"a}ter and M. Beese is gratefully acknowledged. We thank H. G. Tr{\"u}per for his taxonomic and nomenclatural advice. We are especially grateful to the US National Science Foundation (Polar Program) and E. Imre Friedmann (Tallahassee, Florida) for generous support during three antarctic expeditions on his personal grant (DPP83-14180) and for his hospitality in his laboratory. The Deutsche Forschungsgemeinschaft supported P. H. (grants Hi 68/13/1-5).",

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AU - Hirsch, Peter

AU - Gallikowski, Claudia A.

AU - Siebert, Jörg

AU - Peissl, Klaus

AU - Kroppenstedt, Reiner

AU - Schumann, Peter

AU - Stackebrandt, Erko

AU - Anderson, Robert

N1 - Funding Information: Skillful technical assistance of B. Hoffmann, A. Gräter and M. Beese is gratefully acknowledged. We thank H. G. Trüper for his taxonomic and nomenclatural advice. We are especially grateful to the US National Science Foundation (Polar Program) and E. Imre Friedmann (Tallahassee, Florida) for generous support during three antarctic expeditions on his personal grant (DPP83-14180) and for his hospitality in his laboratory. The Deutsche Forschungsgemeinschaft supported P. H. (grants Hi 68/13/1-5).

PY - 2004/11

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N2 - Six Gram-positive, non-motile, UV- and draught-tolerant bacteria were isolated from antarctic soil and rock samples. The pink to orange cocci grew well on oligotrophic medium PYGV (pH 7.5) at 9-18°C. They tolerated 0-10 % NaCl, were aerobic to facultatively anaerobic and contained ornithine in their cell wall (type A3β, Orn-Gly2). The lipid profiles of four strains were found to be typical for those of D. radiodurans. Major fatty acids were 16:1cis9, 15:1cis9, 17:1cis9 and i17:1cis9, the respiratory quinone of three strains was MK-8. Comparative 16S rDNA gene sequencing revealed phylogenetic relationships to the Deinococcus clade, especially to D. radiopugnans. The levels of 16S rRNA gene sequence similarity and DNA-DNA hybridisation data showed the six isolates represented new taxa. Phenotypic properties supported the description of three new species which were different from the eight known Deinococcus species and particularly from D. radiopugnans. Soil isolate AA-692T (DSM 12807T) is the type strain of Deinococcus frigens sp. nov., with AA-752 (DSM 15993) and AA-829 (DSM 15994) as additional strains from soil. The endolithic isolate AA-1444T, Deinococcus saxicola sp. nov., (DSM 15974T) came from antarctic sandstone, and Deinococcus marmoris sp. nov. (isolate AA-63T [DSM 12784T]) as well as AA-69 (DSM 15951) were isolated from antarctic marble.

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Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental antarctica

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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