Abstract
Vascular perturbation is a hallmark of severe forms of dengue disease. We show here that antibodyenhanced dengue virus infection of primary human cord blood-derived mast cells (CBMCs) and the human mast cell-like line HMC-1 results in the release of factor(s) which activate human endothelial cells, as evidenced by increased expression of the adhesion molecules ICAM-1 and VCAM-1. Endothelial cell activation was prevented by pretreatment of mast cell-derived supernatants with a tumor necrosis factor (TNF)-specific blocking antibody, thus identifying TNF as the endothelial cell-activating factor. Our findings suggest that mast cells may represent an important source of TNF, promoting vascular endothelial perturbation following antibody-enhanced dengue virus infection.
Original language | English |
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Pages (from-to) | 1145-1150 |
Number of pages | 6 |
Journal | Journal of Virology |
Volume | 85 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jan 2011 |
ASJC Scopus Subject Areas
- Microbiology
- Immunology
- Insect Science
- Virology