Dengue virus infection of mast cells triggers endothelial cell activation

Michael G. Brown, Laura L. Hermann, Andrew C. Issekutz, Jean S. Marshall, Derek Rowter, Ayham Al-Afif, Robert Anderson

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

Vascular perturbation is a hallmark of severe forms of dengue disease. We show here that antibodyenhanced dengue virus infection of primary human cord blood-derived mast cells (CBMCs) and the human mast cell-like line HMC-1 results in the release of factor(s) which activate human endothelial cells, as evidenced by increased expression of the adhesion molecules ICAM-1 and VCAM-1. Endothelial cell activation was prevented by pretreatment of mast cell-derived supernatants with a tumor necrosis factor (TNF)-specific blocking antibody, thus identifying TNF as the endothelial cell-activating factor. Our findings suggest that mast cells may represent an important source of TNF, promoting vascular endothelial perturbation following antibody-enhanced dengue virus infection.

Original languageEnglish
Pages (from-to)1145-1150
Number of pages6
JournalJournal of Virology
Volume85
Issue number2
DOIs
Publication statusPublished - Jan 2011

ASJC Scopus Subject Areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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