Abstract
1. 1. The presence of DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) has been demonstrated in L cells grown in culture and its properties have been studied. 2. 2. This DNA polymerase has been purified approximately 15-fold with respect to the protein of the crude extracts. 3. 3. The activity of crude extracts as well as that of partially purified preparations has been shown to be almost entirely dependent on the presence of a denatured DNA primer. For full activity of the purified preparation, all four deoxyribonucleoside triphosphates and Mg2+ were also necessary. 4. 4. When DNA synthesis in L cells was inhibited in vivo, the DNA polymerase activity of cell extracts increased. When the inhibition was reversed, and DNA synthesis was reinitiated, the activity of the enzyme decreased. 5. 5. The observed changes in the activity of DNA polymerase are postulated to be due to altered intracellular states of the enzyme.
| Original language | English |
|---|---|
| Pages (from-to) | 193-203 |
| Number of pages | 11 |
| Journal | BBA Specialized Section on Nucleic Acids and Related Subjects |
| Volume | 80 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 17 1964 |
| Externally published | Yes |
Bibliographical note
Funding Information:discussions of this work, and to Mrs. I. Oss for technical assistance. Financial support was received from the National Cancer Institute of Canada and the Medical Research Council of Canada. One of us (M.G.) held a W. P. Caven Memorial Fellowship at the time of this work.
ASJC Scopus Subject Areas
- General Medicine