Development and characterization of 36 novel microsatellite markers for lake chub (Couesius plumbeus) using illumina paired-end sequencing

Gregory R. McCracken; Hilary T. Brewis; Meghan C. McBride; Robert Perry; Donald Keefe; Daniel E. Ruzzante

doi:10.1007/s12686-014-0228-2

Development and characterization of 36 novel microsatellite markers for lake chub (Couesius plumbeus) using illumina paired-end sequencing

Gregory R. McCracken, Hilary T. Brewis, Meghan C. McBride, Robert Perry, Donald Keefe, Daniel E. Ruzzante

Medicine

Research output: Contribution to journal › Letter › peer-review

4 Citations (Scopus)

Abstract

Lake chub is a common freshwater cyprinid found throughout Canada and parts of the northern United States. Here we describe 36 novel microsatellite loci, 25 of which are polymorphic, exhibiting between 2 and 17 alleles each, while the remaining 11 loci are monomorphic. Observed and expected heterozygosities for the polymorphic markers ranged from 0.125 to 0.875 and 0.146 to 0.878, respectively. There was no evidence of linkage disequilibrium between any locus pairs, nor was there any evidence of null alleles. There were however, six markers deviating from Hardy–Weinberg Equilibrium, a result we attribute to relatively small sample size.

Original language	English
Pages (from-to)	873-876
Number of pages	4
Journal	Conservation Genetics Resources
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/s12686-014-0228-2
Publication status	Published - Dec 2014

Bibliographical note

Funding Information:
Acknowledgments We thank Bill Green, Shawn Avery and Lorne Pike for their help collecting and processing samples. Funding for this research was provided by NSERC’s Grant STPG 430198 to DER.

Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.

ASJC Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-014-0228-2

Cite this

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title = "Development and characterization of 36 novel microsatellite markers for lake chub (Couesius plumbeus) using illumina paired-end sequencing",

abstract = "Lake chub is a common freshwater cyprinid found throughout Canada and parts of the northern United States. Here we describe 36 novel microsatellite loci, 25 of which are polymorphic, exhibiting between 2 and 17 alleles each, while the remaining 11 loci are monomorphic. Observed and expected heterozygosities for the polymorphic markers ranged from 0.125 to 0.875 and 0.146 to 0.878, respectively. There was no evidence of linkage disequilibrium between any locus pairs, nor was there any evidence of null alleles. There were however, six markers deviating from Hardy–Weinberg Equilibrium, a result we attribute to relatively small sample size.",

author = "McCracken, {Gregory R.} and Brewis, {Hilary T.} and McBride, {Meghan C.} and Robert Perry and Donald Keefe and Ruzzante, {Daniel E.}",

note = "Funding Information: Acknowledgments We thank Bill Green, Shawn Avery and Lorne Pike for their help collecting and processing samples. Funding for this research was provided by NSERC{\textquoteright}s Grant STPG 430198 to DER. Publisher Copyright: {\textcopyright} 2014, Springer Science+Business Media Dordrecht.",

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doi = "10.1007/s12686-014-0228-2",

language = "English",

volume = "6",

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journal = "Conservation Genetics Resources",

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T1 - Development and characterization of 36 novel microsatellite markers for lake chub (Couesius plumbeus) using illumina paired-end sequencing

AU - McCracken, Gregory R.

AU - Brewis, Hilary T.

AU - McBride, Meghan C.

AU - Perry, Robert

AU - Keefe, Donald

AU - Ruzzante, Daniel E.

N1 - Funding Information: Acknowledgments We thank Bill Green, Shawn Avery and Lorne Pike for their help collecting and processing samples. Funding for this research was provided by NSERC’s Grant STPG 430198 to DER. Publisher Copyright: © 2014, Springer Science+Business Media Dordrecht.

PY - 2014/12

Y1 - 2014/12

N2 - Lake chub is a common freshwater cyprinid found throughout Canada and parts of the northern United States. Here we describe 36 novel microsatellite loci, 25 of which are polymorphic, exhibiting between 2 and 17 alleles each, while the remaining 11 loci are monomorphic. Observed and expected heterozygosities for the polymorphic markers ranged from 0.125 to 0.875 and 0.146 to 0.878, respectively. There was no evidence of linkage disequilibrium between any locus pairs, nor was there any evidence of null alleles. There were however, six markers deviating from Hardy–Weinberg Equilibrium, a result we attribute to relatively small sample size.

AB - Lake chub is a common freshwater cyprinid found throughout Canada and parts of the northern United States. Here we describe 36 novel microsatellite loci, 25 of which are polymorphic, exhibiting between 2 and 17 alleles each, while the remaining 11 loci are monomorphic. Observed and expected heterozygosities for the polymorphic markers ranged from 0.125 to 0.875 and 0.146 to 0.878, respectively. There was no evidence of linkage disequilibrium between any locus pairs, nor was there any evidence of null alleles. There were however, six markers deviating from Hardy–Weinberg Equilibrium, a result we attribute to relatively small sample size.

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Development and characterization of 36 novel microsatellite markers for lake chub (Couesius plumbeus) using illumina paired-end sequencing

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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