Dietary t10,c12-CLA but not c9,t11 CLA Reduces Adipocyte Size in the Absence of Changes in the Adipose Renin-Angiotensin System in fa/fa Zucker Rats

Vanessa Declercq; Peter Zahradka; Carla G. Taylor

doi:10.1007/s11745-010-3469-1

Dietary t10,c12-CLA but not c9,t11 CLA Reduces Adipocyte Size in the Absence of Changes in the Adipose Renin-Angiotensin System in fa/fa Zucker Rats

Vanessa Declercq, Peter Zahradka, Carla G. Taylor

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

In obesity, increased activity of the local renin-angiotensin system (RAS) and enlarged adipocytes with altered adipokine production are linked to the development of obesity-related health problems and cardiovascular disease. Mixtures of conjugated linoleic acid (CLA) isomers have been shown to reduce adipocyte size and alter the production of adipokines. The objective of this study was to investigate the effects of feeding individual CLA isomers on adipocyte size and adipokines associated with the local adipose RAS. Male fa/fa Zucker rats received either (a) control, (b) cis(c)9,trans(t)11-CLA, or (c) t10,c12-CLA diet for 8 weeks. The t10,c12-CLA isomer reduced adipocyte size and increased cell number in epididymal adipose tissue. RT-PCR and Western blot analysis revealed that neither CLA isomer altered mRNA or protein levels of angiotensinogen or AngII receptors in adipose tissue. Likewise, levels of the pro-inflammatory cytokines TNF-α and IL-6 or the anti-inflammatory cytokine IL-10 were unchanged in adipose tissue. Similarly, neither CLA isomer had any effect on phosphorylation nor DNA binding of NF-κB. Our results suggest that although the t10,c12-CLA isomer had beneficial effects on reducing adipocyte size in obese rats, this did not translate into changes in the local adipose RAS or associated adipokines.

Original language	English
Pages (from-to)	1025-1033
Number of pages	9
Journal	Lipids
Volume	45
Issue number	11
DOIs	https://doi.org/10.1007/s11745-010-3469-1
Publication status	Published - Nov 2010
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments We would like to thank Danielle Stringer and Lisa Rigaux for help with the animal care and the contribution of the St. Boniface Hospital and Research Foundation towards infrastructure support. Financial support for this study was provided by grants from Dairy Farmers of Canada (C.T. and P.Z.) and Canada-Manitoba Agri-Food Research and Development Initiative (C.T. and P.Z.), and a Natural Sciences and Engineering Research Council of Canada as postgraduate scholarship (V.D.).

ASJC Scopus Subject Areas

Biochemistry
Organic Chemistry
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11745-010-3469-1

Cite this

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title = "Dietary t10,c12-CLA but not c9,t11 CLA Reduces Adipocyte Size in the Absence of Changes in the Adipose Renin-Angiotensin System in fa/fa Zucker Rats",

abstract = "In obesity, increased activity of the local renin-angiotensin system (RAS) and enlarged adipocytes with altered adipokine production are linked to the development of obesity-related health problems and cardiovascular disease. Mixtures of conjugated linoleic acid (CLA) isomers have been shown to reduce adipocyte size and alter the production of adipokines. The objective of this study was to investigate the effects of feeding individual CLA isomers on adipocyte size and adipokines associated with the local adipose RAS. Male fa/fa Zucker rats received either (a) control, (b) cis(c)9,trans(t)11-CLA, or (c) t10,c12-CLA diet for 8 weeks. The t10,c12-CLA isomer reduced adipocyte size and increased cell number in epididymal adipose tissue. RT-PCR and Western blot analysis revealed that neither CLA isomer altered mRNA or protein levels of angiotensinogen or AngII receptors in adipose tissue. Likewise, levels of the pro-inflammatory cytokines TNF-α and IL-6 or the anti-inflammatory cytokine IL-10 were unchanged in adipose tissue. Similarly, neither CLA isomer had any effect on phosphorylation nor DNA binding of NF-κB. Our results suggest that although the t10,c12-CLA isomer had beneficial effects on reducing adipocyte size in obese rats, this did not translate into changes in the local adipose RAS or associated adipokines.",

author = "Vanessa Declercq and Peter Zahradka and Taylor, {Carla G.}",

note = "Funding Information: Acknowledgments We would like to thank Danielle Stringer and Lisa Rigaux for help with the animal care and the contribution of the St. Boniface Hospital and Research Foundation towards infrastructure support. Financial support for this study was provided by grants from Dairy Farmers of Canada (C.T. and P.Z.) and Canada-Manitoba Agri-Food Research and Development Initiative (C.T. and P.Z.), and a Natural Sciences and Engineering Research Council of Canada as postgraduate scholarship (V.D.).",

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AU - Taylor, Carla G.

N1 - Funding Information: Acknowledgments We would like to thank Danielle Stringer and Lisa Rigaux for help with the animal care and the contribution of the St. Boniface Hospital and Research Foundation towards infrastructure support. Financial support for this study was provided by grants from Dairy Farmers of Canada (C.T. and P.Z.) and Canada-Manitoba Agri-Food Research and Development Initiative (C.T. and P.Z.), and a Natural Sciences and Engineering Research Council of Canada as postgraduate scholarship (V.D.).

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AB - In obesity, increased activity of the local renin-angiotensin system (RAS) and enlarged adipocytes with altered adipokine production are linked to the development of obesity-related health problems and cardiovascular disease. Mixtures of conjugated linoleic acid (CLA) isomers have been shown to reduce adipocyte size and alter the production of adipokines. The objective of this study was to investigate the effects of feeding individual CLA isomers on adipocyte size and adipokines associated with the local adipose RAS. Male fa/fa Zucker rats received either (a) control, (b) cis(c)9,trans(t)11-CLA, or (c) t10,c12-CLA diet for 8 weeks. The t10,c12-CLA isomer reduced adipocyte size and increased cell number in epididymal adipose tissue. RT-PCR and Western blot analysis revealed that neither CLA isomer altered mRNA or protein levels of angiotensinogen or AngII receptors in adipose tissue. Likewise, levels of the pro-inflammatory cytokines TNF-α and IL-6 or the anti-inflammatory cytokine IL-10 were unchanged in adipose tissue. Similarly, neither CLA isomer had any effect on phosphorylation nor DNA binding of NF-κB. Our results suggest that although the t10,c12-CLA isomer had beneficial effects on reducing adipocyte size in obese rats, this did not translate into changes in the local adipose RAS or associated adipokines.

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Dietary t10,c12-CLA but not c9,t11 CLA Reduces Adipocyte Size in the Absence of Changes in the Adipose Renin-Angiotensin System in fa/fa Zucker Rats

Abstract

Bibliographical note

ASJC Scopus Subject Areas

UN SDGs

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