TY - JOUR
T1 - Effect of PEG and mPEG-anthracene on tRNA aggregation and particle formation
AU - Froehlich, E.
AU - Mandeville, J. S.
AU - Arnold, D.
AU - Kreplak, L.
AU - Tajmir-Riahi, H. A.
PY - 2012/1/9
Y1 - 2012/1/9
N2 - Poly(ethylene glycol) (PEG) and its derivatives are synthetic polymers with major applications in gene and drug delivery systems. Synthetic polymers are also used to transport miRNA and siRNA in vitro. We studied the interaction of tRNA with several PEGs of different compositions, such as PEG 3350, PEG 6000, and mPEG-anthracene under physiological conditions. FTIR, UV-visible, CD, and fluorescence spectroscopic methods as well as atomic force microscopy (AFM) were used to analyze the PEG binding mode, the binding constant, and the effects of polymer complexation on tRNA stability, aggregation, and particle formation. Structural analysis showed that PEG-tRNA interaction occurs via RNA bases and the backbone phosphate group with both hydrophilic and hydrophobic contacts. The overall binding constants of K PEG 3350-tRNA= 1.9 (±0.5) × 10 4 M -1, K PEG 6000-tRNA = 8.9 (±1) × 10 4 M -1, and K mPEG-anthracene= 1.2 (±0.40) × 10 3 M -1 show stronger polymer-RNA complexation by PEG 6000 and by PEG 3350 than the mPEG-anthracene. AFM imaging showed that PEG complexes contain on average one tRNA with PEG 3350, five tRNA with PEG 6000, and ten tRNA molecules with mPEG-anthracene. tRNA aggregation and particle formation occurred at high polymer concentrations, whereas it remains in A-family structure.
AB - Poly(ethylene glycol) (PEG) and its derivatives are synthetic polymers with major applications in gene and drug delivery systems. Synthetic polymers are also used to transport miRNA and siRNA in vitro. We studied the interaction of tRNA with several PEGs of different compositions, such as PEG 3350, PEG 6000, and mPEG-anthracene under physiological conditions. FTIR, UV-visible, CD, and fluorescence spectroscopic methods as well as atomic force microscopy (AFM) were used to analyze the PEG binding mode, the binding constant, and the effects of polymer complexation on tRNA stability, aggregation, and particle formation. Structural analysis showed that PEG-tRNA interaction occurs via RNA bases and the backbone phosphate group with both hydrophilic and hydrophobic contacts. The overall binding constants of K PEG 3350-tRNA= 1.9 (±0.5) × 10 4 M -1, K PEG 6000-tRNA = 8.9 (±1) × 10 4 M -1, and K mPEG-anthracene= 1.2 (±0.40) × 10 3 M -1 show stronger polymer-RNA complexation by PEG 6000 and by PEG 3350 than the mPEG-anthracene. AFM imaging showed that PEG complexes contain on average one tRNA with PEG 3350, five tRNA with PEG 6000, and ten tRNA molecules with mPEG-anthracene. tRNA aggregation and particle formation occurred at high polymer concentrations, whereas it remains in A-family structure.
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U2 - 10.1021/bm2016515
DO - 10.1021/bm2016515
M3 - Article
C2 - 22176277
AN - SCOPUS:84855568328
SN - 1525-7797
VL - 13
SP - 282
EP - 287
JO - Biomacromolecules
JF - Biomacromolecules
IS - 1
ER -