Abstract
Chemerin is a novel adipokine associated with obesity and insulin resistance. α-Lipoic acid (α-LA) has shown beneficial properties on diabetes and obesity. The aim of this study was to examine the effects of α-LA on chemerin production in adipocytes in absence or presence of TNF-α, insulin and AICAR. The potential signaling pathways involved in α-LA effects on chemerin were also analyzed. α-LA actions on chemerin were tested in differentiated 3T3-L1 adipocytes and in some cases in human subcutaneous and omental adipocytes. Chemerin mRNA levels were measured by RT-PCR and the amount of chemerin secreted to culture media was determined by ELISA. α-LA induced a concentration-dependent inhibition on both chemerin secretion and mRNA levels in 3T3-L1 adipocytes. The AMPK activator AICAR and the PI3K inhibitor LY294002 dramatically abrogated both chemerin secretion and gene expression, and further potentiated the inhibitory effect of α-LA on chemerin secretion. Insulin was able to partially reverse the inhibitory action of α-LA on chemerin secretion. α-LA also reduced basal chemerin secretion in both subcutaneous and omental adipocytes from overweight/obese subjects. Moreover, α-LA was able to abolish the stimulatory effects of the pro-inflammatory cytokine TNF-α on chemerin secretion. Our data demonstrated the ability of α-LA to inhibit chemerin production, an adipokine associated to obesity and metabolic syndrome, suggesting that the reduction of chemerin could contribute to the antiobesity/antidiabetic properties described for α-LA.
Original language | English |
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Pages (from-to) | 260-268 |
Number of pages | 9 |
Journal | Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids |
Volume | 1861 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 1 2016 |
Bibliographical note
Funding Information:This work has been supported by the Ministry of Science and Innovation of the Government of Spain ( AGL 2009-10,873/ALI ) and by Línea Especial: “Nutrición, Obesidad y Salud” (LE/97 University of Navarra) and by the Canadian Institutes of Health Research. PL Prieto-Hontoria was supported by a research grant by Instituto Danone, Spain. M. Fernández-Galilea was supported by a predoctoral grant from Navarra Goverment. M. López-Yoldi was supported by a predoctoral grant from Asociación de Amigos de la Universidad de Navarra.
Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
ASJC Scopus Subject Areas
- Molecular Biology
- Cell Biology