Effects of ionophoretically applied bombesin-like peptides on hamster suprachiasmatic nucleus neurons in vitro

Hugh D. Piggins; David J. Cutler; Benjamin Rusak

doi:10.1016/0014-2999(94)90801-X

Effects of ionophoretically applied bombesin-like peptides on hamster suprachiasmatic nucleus neurons in vitro

Hugh D. Piggins, David J. Cutler, Benjamin Rusak

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Ionophoresis of the smaller bombesin-like peptides (gastrin releasing peptide [GRP]-(18-27), neuromedin B, and bombesin) evoked responses from 30-60% of hamster suprachiasmatic nucleus cells recorded in a hypothalamic slice preparation, depending on the circadian phase. We also demonstrated for the first time that the putative bombesin-like peptide receptor antagonists [D-F₅,D-Phe⁶, D-Ala¹¹]bombesin-(6-13)methyl ester (BIM 26226) and [D-Phe⁶,Des-Met¹⁴]bombesin- (6-14)ethyl amide can be applied ionophoretically to block physiological responses to bombesin-like peptides. Together with earlier findings, these results show that bombesin-like peptides administered by several methods can potently alter the firing rates of hamster suprachiasmatic nucleus neurons in vitro. These results indicate that bombesin-like peptides affect suprachiasmatic nucleus cells and could play a role in modulating suprachiasmatic nucleus-mediated circadian rhythm entrainment.

Original language	English
Pages (from-to)	413-419
Number of pages	7
Journal	European Journal of Pharmacology
Volume	271
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-2999(94)90801-X
Publication status	Published - Dec 27 1994

Bibliographical note

Funding Information:
This research was supported by grants from the US Air Force Office of Scientific Research (F49620-93-1-0089), and NSERC of Canada (A0305). H.D.P. was supported by postdoctoral fellowships from NSERC and MRC of Canada. We are grateful to Dr. John Taylor (Biomeasure Inc.) for his gift of BIM 26226.

ASJC Scopus Subject Areas

Pharmacology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0014-2999(94)90801-X

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title = "Effects of ionophoretically applied bombesin-like peptides on hamster suprachiasmatic nucleus neurons in vitro",

abstract = "Ionophoresis of the smaller bombesin-like peptides (gastrin releasing peptide [GRP]-(18-27), neuromedin B, and bombesin) evoked responses from 30-60% of hamster suprachiasmatic nucleus cells recorded in a hypothalamic slice preparation, depending on the circadian phase. We also demonstrated for the first time that the putative bombesin-like peptide receptor antagonists [D-F5,D-Phe6, D-Ala11]bombesin-(6-13)methyl ester (BIM 26226) and [D-Phe6,Des-Met14]bombesin- (6-14)ethyl amide can be applied ionophoretically to block physiological responses to bombesin-like peptides. Together with earlier findings, these results show that bombesin-like peptides administered by several methods can potently alter the firing rates of hamster suprachiasmatic nucleus neurons in vitro. These results indicate that bombesin-like peptides affect suprachiasmatic nucleus cells and could play a role in modulating suprachiasmatic nucleus-mediated circadian rhythm entrainment.",

author = "Piggins, {Hugh D.} and Cutler, {David J.} and Benjamin Rusak",

note = "Funding Information: This research was supported by grants from the US Air Force Office of Scientific Research (F49620-93-1-0089), and NSERC of Canada (A0305). H.D.P. was supported by postdoctoral fellowships from NSERC and MRC of Canada. We are grateful to Dr. John Taylor (Biomeasure Inc.) for his gift of BIM 26226.",

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AU - Cutler, David J.

AU - Rusak, Benjamin

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PY - 1994/12/27

Y1 - 1994/12/27

N2 - Ionophoresis of the smaller bombesin-like peptides (gastrin releasing peptide [GRP]-(18-27), neuromedin B, and bombesin) evoked responses from 30-60% of hamster suprachiasmatic nucleus cells recorded in a hypothalamic slice preparation, depending on the circadian phase. We also demonstrated for the first time that the putative bombesin-like peptide receptor antagonists [D-F5,D-Phe6, D-Ala11]bombesin-(6-13)methyl ester (BIM 26226) and [D-Phe6,Des-Met14]bombesin- (6-14)ethyl amide can be applied ionophoretically to block physiological responses to bombesin-like peptides. Together with earlier findings, these results show that bombesin-like peptides administered by several methods can potently alter the firing rates of hamster suprachiasmatic nucleus neurons in vitro. These results indicate that bombesin-like peptides affect suprachiasmatic nucleus cells and could play a role in modulating suprachiasmatic nucleus-mediated circadian rhythm entrainment.

AB - Ionophoresis of the smaller bombesin-like peptides (gastrin releasing peptide [GRP]-(18-27), neuromedin B, and bombesin) evoked responses from 30-60% of hamster suprachiasmatic nucleus cells recorded in a hypothalamic slice preparation, depending on the circadian phase. We also demonstrated for the first time that the putative bombesin-like peptide receptor antagonists [D-F5,D-Phe6, D-Ala11]bombesin-(6-13)methyl ester (BIM 26226) and [D-Phe6,Des-Met14]bombesin- (6-14)ethyl amide can be applied ionophoretically to block physiological responses to bombesin-like peptides. Together with earlier findings, these results show that bombesin-like peptides administered by several methods can potently alter the firing rates of hamster suprachiasmatic nucleus neurons in vitro. These results indicate that bombesin-like peptides affect suprachiasmatic nucleus cells and could play a role in modulating suprachiasmatic nucleus-mediated circadian rhythm entrainment.

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Effects of ionophoretically applied bombesin-like peptides on hamster suprachiasmatic nucleus neurons in vitro

Abstract

Bibliographical note

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PubMed: MeSH publication types

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