Emerging Biotechnology Applications of Aqueous Two-Phase Systems

Alyne G. Teixeira; Rishima Agarwal; Kristin Robin Ko; Jessica Grant-Burt; Brendan M. Leung; John P. Frampton

doi:10.1002/adhm.201701036

Emerging Biotechnology Applications of Aqueous Two-Phase Systems

Alyne G. Teixeira, Rishima Agarwal, Kristin Robin Ko, Jessica Grant-Burt, Brendan M. Leung, John P. Frampton

Medicine

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

Liquid–liquid phase separation between aqueous solutions containing two incompatible polymers, a polymer and a salt, or a polymer and a surfactant, has been exploited for a wide variety of biotechnology applications throughout the years. While many applications for aqueous two-phase systems fall within the realm of separation science, the ability to partition many different materials within these systems, coupled with recent advances in materials science and liquid handling, has allowed bioengineers to imagine new applications. This progress report provides an overview of the history and key properties of aqueous two-phase systems to lend context to how these materials have progressed to modern applications such as cellular micropatterning and bioprinting, high-throughput 3D tissue assembly, microscale biomolecular assay development, facilitation of cell separation and microcapsule production using microfluidic devices, and synthetic biology. Future directions and present limitations and design considerations of this adaptable and promising toolkit for biomolecule and cellular manipulation are further evaluated.

Original language	English
Article number	1701036
Journal	Advanced healthcare materials
Volume	7
Issue number	6
DOIs	https://doi.org/10.1002/adhm.201701036
Publication status	Published - Mar 21 2018

Bibliographical note

Funding Information:
A.G.T. wishes to acknowledge support from the Nova Scotia Provincial Government (NSGS Doctoral Scholarship). R.A. wishes to acknowledge scholarships from the Canadian Institutes of Health Research (CGS) and the Dalhousie University Faculty of Engineering (Exxon Mobil Canada Ltd.). K.R.K. wishes to acknowledge scholarships from the Canadian Institutes of Health Research (CGS), Nova Scotia Health Research Foundation (Scotia Scholar), and the Nova Scotia Provincial Government (NSGS). This work was supported by funds from the Canada Research Chairs Program, Canada Foundation for Innovation (Project #33533), and the Natural Sciences and Engineering Research Council of Canada (RGPIN-2016-04298).

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

ASJC Scopus Subject Areas

Biomaterials
Biomedical Engineering
Pharmaceutical Science

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10.1002/adhm.201701036

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Teixeira, A. G., Agarwal, R., Ko, K. R., Grant-Burt, J., Leung, B. M., & Frampton, J. P. (2018). Emerging Biotechnology Applications of Aqueous Two-Phase Systems. Advanced healthcare materials, 7(6), Article 1701036. https://doi.org/10.1002/adhm.201701036

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Emerging Biotechnology Applications of Aqueous Two-Phase Systems

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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