Expression of fosB mRNA in the hamster suprachiasmatic nucleus is inducted at only selected circadian phases

Mario E. Guido; Benjamin Rusak; Harold A. Robertson

doi:10.1016/S0006-8993(96)00816-5

Expression of fosB mRNA in the hamster suprachiasmatic nucleus is inducted at only selected circadian phases

Mario E. Guido, Benjamin Rusak, Harold A. Robertson

Medicine

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We have studied the expression of fosB mRNA in the suprachiasmatic nucleus (SCN) of hamsters by in situ hybridization using oligonucleotides with sequences complementary to the C-terminal of the fosB mRNA sequence. In animals exposed for 48 h to darkness, there was little or no background expression in SCN cells of fosB mRNA at any circadian phase. Light pulses (30 min) were able to induce fosB expression only during the subjective night. Transcripts of fosB increased rapidly to peak by the end of a 30-min light pulse. Light-induced increases gradually declined in darkness, but levels were still elevated for up to 150 min after the light pulse. Induction in response to a light pulse was largely restricted to the ventrolateral portion of the nucleus which receives the heaviest retinal projection. The temporal and anatomical pattern of fosB mRNA expression in the hamster SCN therefore resembles that reported previously for other immediate-early genes, such as c-fos.

Original language	English
Pages (from-to)	132-138
Number of pages	7
Journal	Brain Research
Volume	739
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(96)00816-5
Publication status	Published - Nov 11 1996

Bibliographical note

Funding Information:
We are grateful to Donna Goguen, Victoria Muise, Kay Murphy and Marc Peterson for their excellent technical assistance and advice. Supported by grants from the Medical Research Council of Canada (MA8929; MT10644), and a postdoctoral fellowship (M.E.G.) from CONICET of Argentina.

ASJC Scopus Subject Areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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Guido, M. E., Rusak, B., & Robertson, H. A. (1996). Expression of fosB mRNA in the hamster suprachiasmatic nucleus is inducted at only selected circadian phases. Brain Research, 739(1-2), 132-138. https://doi.org/10.1016/S0006-8993(96)00816-5

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abstract = "We have studied the expression of fosB mRNA in the suprachiasmatic nucleus (SCN) of hamsters by in situ hybridization using oligonucleotides with sequences complementary to the C-terminal of the fosB mRNA sequence. In animals exposed for 48 h to darkness, there was little or no background expression in SCN cells of fosB mRNA at any circadian phase. Light pulses (30 min) were able to induce fosB expression only during the subjective night. Transcripts of fosB increased rapidly to peak by the end of a 30-min light pulse. Light-induced increases gradually declined in darkness, but levels were still elevated for up to 150 min after the light pulse. Induction in response to a light pulse was largely restricted to the ventrolateral portion of the nucleus which receives the heaviest retinal projection. The temporal and anatomical pattern of fosB mRNA expression in the hamster SCN therefore resembles that reported previously for other immediate-early genes, such as c-fos.",

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note = "Funding Information: We are grateful to Donna Goguen, Victoria Muise, Kay Murphy and Marc Peterson for their excellent technical assistance and advice. Supported by grants from the Medical Research Council of Canada (MA8929; MT10644), and a postdoctoral fellowship (M.E.G.) from CONICET of Argentina.",

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AU - Rusak, Benjamin

AU - Robertson, Harold A.

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N2 - We have studied the expression of fosB mRNA in the suprachiasmatic nucleus (SCN) of hamsters by in situ hybridization using oligonucleotides with sequences complementary to the C-terminal of the fosB mRNA sequence. In animals exposed for 48 h to darkness, there was little or no background expression in SCN cells of fosB mRNA at any circadian phase. Light pulses (30 min) were able to induce fosB expression only during the subjective night. Transcripts of fosB increased rapidly to peak by the end of a 30-min light pulse. Light-induced increases gradually declined in darkness, but levels were still elevated for up to 150 min after the light pulse. Induction in response to a light pulse was largely restricted to the ventrolateral portion of the nucleus which receives the heaviest retinal projection. The temporal and anatomical pattern of fosB mRNA expression in the hamster SCN therefore resembles that reported previously for other immediate-early genes, such as c-fos.

AB - We have studied the expression of fosB mRNA in the suprachiasmatic nucleus (SCN) of hamsters by in situ hybridization using oligonucleotides with sequences complementary to the C-terminal of the fosB mRNA sequence. In animals exposed for 48 h to darkness, there was little or no background expression in SCN cells of fosB mRNA at any circadian phase. Light pulses (30 min) were able to induce fosB expression only during the subjective night. Transcripts of fosB increased rapidly to peak by the end of a 30-min light pulse. Light-induced increases gradually declined in darkness, but levels were still elevated for up to 150 min after the light pulse. Induction in response to a light pulse was largely restricted to the ventrolateral portion of the nucleus which receives the heaviest retinal projection. The temporal and anatomical pattern of fosB mRNA expression in the hamster SCN therefore resembles that reported previously for other immediate-early genes, such as c-fos.

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Expression of fosB mRNA in the hamster suprachiasmatic nucleus is inducted at only selected circadian phases

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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