Fate of modular cardiac tissue constructs in a syngeneic rat model

Brendan M. Leung, Yasuo Miyagi, Ren Ke Li, Michael V. Sefton

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Modular cardiac tissues developed both vascular and cardiac structuresin vivo, provided that the host response was attenuated by omitting xenoproteins from the modules. Collagen gel modules (with MatrigelTM) containing cardiomyocytes (CMs) alone or CMs with surface-seeded endothelial cells (ECs; CM/EC modules) were injected into the peri-infarct zone of the heart in syngeneic Lewis rats. After 3 weeks, donor ECs developed into blood vessel-like structures that also contained erythrocytes. However, no donor CMs were found within the implant sites, presumably because host cells including macrophages and T cells infiltrated extensively into the injection sites. To lessen the host response, Matrigel was omitted from the matrix and the modules were rinsed with serum-free medium prior to implantation. Host cell infiltration was attenuated, resulting in a higher degree of vascularization with CM/EC modules than with CM modules without ECs. Most importantly, donor CMs matured into striated muscle-like structures in Matrigel-free implants.

Original languageEnglish
Pages (from-to)1247-1258
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
& Sons, Ltd.

ASJC Scopus Subject Areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'Fate of modular cardiac tissue constructs in a syngeneic rat model'. Together they form a unique fingerprint.

Cite this

Leung, B. M., Miyagi, Y., Li, R. K., & Sefton, M. V. (2015). Fate of modular cardiac tissue constructs in a syngeneic rat model. Journal of Tissue Engineering and Regenerative Medicine, 9(11), 1247-1258. https://doi.org/10.1002/term.1724