GABA and baclofen potentiate the K+-evoked release of methionine-enkephalin from rat striatal slices

Jana Sawynok; Frank S. Labella

doi:10.1016/0014-2999(81)90204-1

GABA and baclofen potentiate the K⁺-evoked release of methionine-enkephalin from rat striatal slices

Jana Sawynok, Frank S. Labella

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

GABA potentiates the potassium-evoked release of methionine-enkephalin (ME) from slices of rat corpus striatum. This potentiation is observed only when a submaximal concentration (30 mM) of K⁺ is used to evoke release. The effect of GABA is dose-dependent between 100 and 1000 μM. The basal release of ME is not altered by these concentrations of GABA. Baclofen, but not muscimol, mimics the effect of GABA on the evoked release of ME. This effect is not stereoselective as both the (+)- and (-)-isomers of baclofen enhance ME release. Picrotoxin (100 μM) blocks the enhancement of ME release produced by both GABA and baclofen. Bicuculline methiodide (100 μM) does not block the effect of GABA. The effect of GABA on ME release may be mediated by an atypical GABA receptor which is activated by baclofen.

Original language	English
Pages (from-to)	103-110
Number of pages	8
Journal	European Journal of Pharmacology
Volume	70
Issue number	2
DOIs	https://doi.org/10.1016/0014-2999(81)90204-1
Publication status	Published - Mar 12 1981
Externally published	Yes

Bibliographical note

Funding Information:
This work was supportedb y the MedicalR esearch Council of Canada.J .S. is the recipiento f an MRC Fellowship.F .S.L. is an MRC CareerI nvestigatoWr. e thank Ciba-Geigyf or its generoussu pplyof baclofen and its isomers.

ASJC Scopus Subject Areas

Pharmacology

Access to Document

10.1016/0014-2999(81)90204-1

Cite this

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title = "GABA and baclofen potentiate the K+-evoked release of methionine-enkephalin from rat striatal slices",

abstract = "GABA potentiates the potassium-evoked release of methionine-enkephalin (ME) from slices of rat corpus striatum. This potentiation is observed only when a submaximal concentration (30 mM) of K+ is used to evoke release. The effect of GABA is dose-dependent between 100 and 1000 μM. The basal release of ME is not altered by these concentrations of GABA. Baclofen, but not muscimol, mimics the effect of GABA on the evoked release of ME. This effect is not stereoselective as both the (+)- and (-)-isomers of baclofen enhance ME release. Picrotoxin (100 μM) blocks the enhancement of ME release produced by both GABA and baclofen. Bicuculline methiodide (100 μM) does not block the effect of GABA. The effect of GABA on ME release may be mediated by an atypical GABA receptor which is activated by baclofen.",

author = "Jana Sawynok and Labella, {Frank S.}",

note = "Funding Information: This work was supportedb y the MedicalR esearch Council of Canada.J .S. is the recipiento f an MRC Fellowship.F .S.L. is an MRC CareerI nvestigatoWr. e thank Ciba-Geigyf or its generoussu pplyof baclofen and its isomers.",

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AU - Labella, Frank S.

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PY - 1981/3/12

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N2 - GABA potentiates the potassium-evoked release of methionine-enkephalin (ME) from slices of rat corpus striatum. This potentiation is observed only when a submaximal concentration (30 mM) of K+ is used to evoke release. The effect of GABA is dose-dependent between 100 and 1000 μM. The basal release of ME is not altered by these concentrations of GABA. Baclofen, but not muscimol, mimics the effect of GABA on the evoked release of ME. This effect is not stereoselective as both the (+)- and (-)-isomers of baclofen enhance ME release. Picrotoxin (100 μM) blocks the enhancement of ME release produced by both GABA and baclofen. Bicuculline methiodide (100 μM) does not block the effect of GABA. The effect of GABA on ME release may be mediated by an atypical GABA receptor which is activated by baclofen.

AB - GABA potentiates the potassium-evoked release of methionine-enkephalin (ME) from slices of rat corpus striatum. This potentiation is observed only when a submaximal concentration (30 mM) of K+ is used to evoke release. The effect of GABA is dose-dependent between 100 and 1000 μM. The basal release of ME is not altered by these concentrations of GABA. Baclofen, but not muscimol, mimics the effect of GABA on the evoked release of ME. This effect is not stereoselective as both the (+)- and (-)-isomers of baclofen enhance ME release. Picrotoxin (100 μM) blocks the enhancement of ME release produced by both GABA and baclofen. Bicuculline methiodide (100 μM) does not block the effect of GABA. The effect of GABA on ME release may be mediated by an atypical GABA receptor which is activated by baclofen.

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