Green synthesized Ag-nanoparticles using Scutellaria multicaulis stem extract and their selective cytotoxicity against breast cancer

Zahra Gharari, Parichehr Hanachi, Tony R. Walker

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Scutellaria multicaulis is a medicinal herb which has been extensively prescribed in Iranian traditional medicine for treatment of infections, constipation, wounds, and also as medication for anxiety. To evaluate biological activities of biosynthesized silver nanoparticles (Sm-AgNPs), Sm-AgNPswere synthesized using S. multicaulis stem extract as capping and reducing agent. Characterization studies using UV–vis, XRD, DLS, Zeta potential, FESEM, EDX, TEM, FTIR, RAMAN and TGA assays indicated that Sm-AgNPs had optimal shape, size (∼60 nm) and stability (−46.4 mV) to be used as a drug. Findings also demonstrated that Sm-AgNPs display higher Total Phenolics and Total Flavonoids Content and possesses higher antioxidant activity. HPLC-MSn analysis of constituents in the S. multicaulis stem extract and Sm-AgNPs-free supernatant in negative ion mode showed that flavonoids, mainly jaceidin, skullcapflavon II, wogonin, oroxylin A and dihydroxy, trimethoxyflavone from extract have contributed to the synthesis of Sm-AgNPs. Additionally, Sm-AgNPs demonstrated effective anticarcinogenic properties on MDA-MB231 cells proliferation with IC50 value of 81.2 μg/mL at 48 h by inducing cell apoptosis. Overall, results confirmed the potential role of S. multicaulis stem extract as a potential raw material for synthesis of biologically active Sm-AgNPs, for development of new antitumor agents which could be utilized to combat breast cancer.

Original languageEnglish
Article number114786
JournalAnalytical Biochemistry
Volume653
DOIs
Publication statusPublished - Sept 15 2022

Bibliographical note

Funding Information:
This work has been supported financially by Alzahra University , Tehran, Iran, under a postdoctoral scholar (Grant No. 500-3-589 ).

Publisher Copyright:
© 2022 Elsevier Inc.

ASJC Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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