Immune biomarkers predictive of respiratory viral infection in elderly nursing home residents

Jennie Johnstone; Robin Parsons; Fernando Botelho; Jamie Millar; Shelly McNeil; Tamas Fulop; Janet McElhaney; Melissa K. Andrew; Stephen D. Walter; P. J. Devereaux; Mehrnoush Malekesmaeili; Ryan R. Brinkman; James Mahony; Jonathan Bramson; Mark Loeb

doi:10.1371/journal.pone.0108481

Immune biomarkers predictive of respiratory viral infection in elderly nursing home residents

Jennie Johnstone, Robin Parsons, Fernando Botelho, Jamie Millar, Shelly McNeil, Tamas Fulop, Janet McElhaney, Melissa K. Andrew, Stephen D. Walter, P. J. Devereaux, Mehrnoush Malekesmaeili, Ryan R. Brinkman, James Mahony, Jonathan Bramson, Mark Loeb

Medicine

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Methods: Residents ≥65 years from 32 nursing homes in 4 Canadian cities were enrolled in Fall 2009, 2010 and 2011, and followed for one influenza season. Following influenza vaccination, peripheral blood mononuclear cells (PBMCs) were obtained and analysed by flow cytometry for T-regs, CD4+ and CD8+ T-cell subsets (CCR7+CD45RA+, CCR7-CD45RA+ and CD28-CD57+) and CMV-reactive CD4+ and CD8+ T-cells. Nasopharyngeal swabs were obtained and tested for viruses in symptomatic residents. A Cox proportional hazards model adjusted for age, sex and frailty, determined the relationship between immune phenotypes and time to viral infection.

Results:1072 residents were enrolled; median age 86 years and 72% female. 269 swabs were obtained, 87 were positive for virus: influenza (24%), RSV (14%), coronavirus (32%), rhinovirus (17%), human metapneumovirus (9%) and parainfluenza (5%). In multivariable analysis, high T-reg% (HR 0.41, 95% CI 0.20-0.81) and high CMV-reactive CD4+ T-cell% (HR 1.69, 95% CI 1.03-2.78) were predictive of respiratory viral infection.

Conclusions: In elderly nursing home residents, high CMV-reactive CD4+ T-cells were associated with an increased risk and high T-regs were associated with a reduced risk of respiratory viral infection.

Objective: To determine if immune phenotypes associated with immunosenescence predict risk of respiratory viral infection in elderly nursing home residents.

Original language	English
Article number	e108481
Journal	PLoS One
Volume	9
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0108481
Publication status	Published - Oct 2 2014

Bibliographical note

Publisher Copyright:
© 2014 Johnstone et al.

ASJC Scopus Subject Areas

General Biochemistry,Genetics and Molecular Biology
General Agricultural and Biological Sciences
General

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1371/journal.pone.0108481

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Johnstone, J., Parsons, R., Botelho, F., Millar, J., McNeil, S., Fulop, T., McElhaney, J., Andrew, M. K., Walter, S. D., Devereaux, P. J., Malekesmaeili, M., Brinkman, R. R., Mahony, J., Bramson, J., & Loeb, M. (2014). Immune biomarkers predictive of respiratory viral infection in elderly nursing home residents. PLoS One, 9(10), Article e108481. https://doi.org/10.1371/journal.pone.0108481

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title = "Immune biomarkers predictive of respiratory viral infection in elderly nursing home residents",

abstract = "Methods: Residents ≥65 years from 32 nursing homes in 4 Canadian cities were enrolled in Fall 2009, 2010 and 2011, and followed for one influenza season. Following influenza vaccination, peripheral blood mononuclear cells (PBMCs) were obtained and analysed by flow cytometry for T-regs, CD4+ and CD8+ T-cell subsets (CCR7+CD45RA+, CCR7-CD45RA+ and CD28-CD57+) and CMV-reactive CD4+ and CD8+ T-cells. Nasopharyngeal swabs were obtained and tested for viruses in symptomatic residents. A Cox proportional hazards model adjusted for age, sex and frailty, determined the relationship between immune phenotypes and time to viral infection.Results:1072 residents were enrolled; median age 86 years and 72% female. 269 swabs were obtained, 87 were positive for virus: influenza (24%), RSV (14%), coronavirus (32%), rhinovirus (17%), human metapneumovirus (9%) and parainfluenza (5%). In multivariable analysis, high T-reg% (HR 0.41, 95% CI 0.20-0.81) and high CMV-reactive CD4+ T-cell% (HR 1.69, 95% CI 1.03-2.78) were predictive of respiratory viral infection.Conclusions: In elderly nursing home residents, high CMV-reactive CD4+ T-cells were associated with an increased risk and high T-regs were associated with a reduced risk of respiratory viral infection.Objective: To determine if immune phenotypes associated with immunosenescence predict risk of respiratory viral infection in elderly nursing home residents.",

author = "Jennie Johnstone and Robin Parsons and Fernando Botelho and Jamie Millar and Shelly McNeil and Tamas Fulop and Janet McElhaney and Andrew, {Melissa K.} and Walter, {Stephen D.} and Devereaux, {P. J.} and Mehrnoush Malekesmaeili and Brinkman, {Ryan R.} and James Mahony and Jonathan Bramson and Mark Loeb",

note = "Publisher Copyright: {\textcopyright} 2014 Johnstone et al.",

year = "2014",

month = oct,

day = "2",

doi = "10.1371/journal.pone.0108481",

language = "English",

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T1 - Immune biomarkers predictive of respiratory viral infection in elderly nursing home residents

AU - Johnstone, Jennie

AU - Parsons, Robin

AU - Botelho, Fernando

AU - Millar, Jamie

AU - McNeil, Shelly

AU - Fulop, Tamas

AU - McElhaney, Janet

AU - Andrew, Melissa K.

AU - Walter, Stephen D.

AU - Devereaux, P. J.

AU - Malekesmaeili, Mehrnoush

AU - Brinkman, Ryan R.

AU - Mahony, James

AU - Bramson, Jonathan

AU - Loeb, Mark

PY - 2014/10/2

Y1 - 2014/10/2

N2 - Methods: Residents ≥65 years from 32 nursing homes in 4 Canadian cities were enrolled in Fall 2009, 2010 and 2011, and followed for one influenza season. Following influenza vaccination, peripheral blood mononuclear cells (PBMCs) were obtained and analysed by flow cytometry for T-regs, CD4+ and CD8+ T-cell subsets (CCR7+CD45RA+, CCR7-CD45RA+ and CD28-CD57+) and CMV-reactive CD4+ and CD8+ T-cells. Nasopharyngeal swabs were obtained and tested for viruses in symptomatic residents. A Cox proportional hazards model adjusted for age, sex and frailty, determined the relationship between immune phenotypes and time to viral infection.Results:1072 residents were enrolled; median age 86 years and 72% female. 269 swabs were obtained, 87 were positive for virus: influenza (24%), RSV (14%), coronavirus (32%), rhinovirus (17%), human metapneumovirus (9%) and parainfluenza (5%). In multivariable analysis, high T-reg% (HR 0.41, 95% CI 0.20-0.81) and high CMV-reactive CD4+ T-cell% (HR 1.69, 95% CI 1.03-2.78) were predictive of respiratory viral infection.Conclusions: In elderly nursing home residents, high CMV-reactive CD4+ T-cells were associated with an increased risk and high T-regs were associated with a reduced risk of respiratory viral infection.Objective: To determine if immune phenotypes associated with immunosenescence predict risk of respiratory viral infection in elderly nursing home residents.

AB - Methods: Residents ≥65 years from 32 nursing homes in 4 Canadian cities were enrolled in Fall 2009, 2010 and 2011, and followed for one influenza season. Following influenza vaccination, peripheral blood mononuclear cells (PBMCs) were obtained and analysed by flow cytometry for T-regs, CD4+ and CD8+ T-cell subsets (CCR7+CD45RA+, CCR7-CD45RA+ and CD28-CD57+) and CMV-reactive CD4+ and CD8+ T-cells. Nasopharyngeal swabs were obtained and tested for viruses in symptomatic residents. A Cox proportional hazards model adjusted for age, sex and frailty, determined the relationship between immune phenotypes and time to viral infection.Results:1072 residents were enrolled; median age 86 years and 72% female. 269 swabs were obtained, 87 were positive for virus: influenza (24%), RSV (14%), coronavirus (32%), rhinovirus (17%), human metapneumovirus (9%) and parainfluenza (5%). In multivariable analysis, high T-reg% (HR 0.41, 95% CI 0.20-0.81) and high CMV-reactive CD4+ T-cell% (HR 1.69, 95% CI 1.03-2.78) were predictive of respiratory viral infection.Conclusions: In elderly nursing home residents, high CMV-reactive CD4+ T-cells were associated with an increased risk and high T-regs were associated with a reduced risk of respiratory viral infection.Objective: To determine if immune phenotypes associated with immunosenescence predict risk of respiratory viral infection in elderly nursing home residents.

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SN - 1932-6203

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Immune biomarkers predictive of respiratory viral infection in elderly nursing home residents

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

UN SDGs

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