Immunoblotting conditions for small peptides from streptococci

Lauren Davey; Scott A. Halperin; Song F. Lee

doi:10.1016/j.mimet.2015.04.015

Immunoblotting conditions for small peptides from streptococci

Lauren Davey, Scott A. Halperin, Song F. Lee

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Streptococci secrete small peptides with important biological functions. These peptides are not amenable to standard immunoblotting, and are often detected indirectly using activity assays, or by alternative approaches that may be expensive and laborious. Here we describe an immunoblotting method that enables reproducible detection of these small streptococcal peptides.

Original language	English
Pages (from-to)	40-42
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	114
DOIs	https://doi.org/10.1016/j.mimet.2015.04.015
Publication status	Published - Jul 1 2015

Bibliographical note

Funding Information:
Funding for this study was provided by the Natural Sciences and Engineering Research Council of Canada (NSERC) ( 183712-2013 ). LD is a recipient of a NSERC post-graduate scholarship, an IWK graduate scholarship, and a Scotia Scholars Award.

Publisher Copyright:
© 2015 Elsevier B.V.

ASJC Scopus Subject Areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2015.04.015

Cite this

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abstract = "Streptococci secrete small peptides with important biological functions. These peptides are not amenable to standard immunoblotting, and are often detected indirectly using activity assays, or by alternative approaches that may be expensive and laborious. Here we describe an immunoblotting method that enables reproducible detection of these small streptococcal peptides.",

author = "Lauren Davey and Halperin, {Scott A.} and Lee, {Song F.}",

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AU - Halperin, Scott A.

AU - Lee, Song F.

N1 - Funding Information: Funding for this study was provided by the Natural Sciences and Engineering Research Council of Canada (NSERC) ( 183712-2013 ). LD is a recipient of a NSERC post-graduate scholarship, an IWK graduate scholarship, and a Scotia Scholars Award. Publisher Copyright: © 2015 Elsevier B.V.

PY - 2015/7/1

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N2 - Streptococci secrete small peptides with important biological functions. These peptides are not amenable to standard immunoblotting, and are often detected indirectly using activity assays, or by alternative approaches that may be expensive and laborious. Here we describe an immunoblotting method that enables reproducible detection of these small streptococcal peptides.

AB - Streptococci secrete small peptides with important biological functions. These peptides are not amenable to standard immunoblotting, and are often detected indirectly using activity assays, or by alternative approaches that may be expensive and laborious. Here we describe an immunoblotting method that enables reproducible detection of these small streptococcal peptides.

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JF - Journal of Microbiological Methods

ER -

Immunoblotting conditions for small peptides from streptococci

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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