Abstract
Purified bovine milk proteins that were added to cultures of murine spleen cells significantly increased cell proliferation and production of immunoglobulin M. Casein and a whey mixture consisting of α-lactalbumin, bovine serum albumin, bovine gamma globulin, and β-lactoglobulin (β-LG) were stimulatory. Of the three β-LG preparations that are commercially available (β-LG containing variants A and B, purified variant A, and purified variant B), the unseparated mixture containing both the A and B variants showed the most immunomodulatory activity. Both alkaline treatment and trypsin digestion of the β-LG preparation markedly reduced its effectiveness. Polymyxin B, while greatly diminishing the stimulatory effect of lipopolysaccharide, had no significant effect on either the enhancement of cell proliferation or the enhancement of immunoglobulin production by β-LG. In the presence of S-(n-butyl)-homocysteine sulfoxamine, the stimulatory effect of β-LG on cell proliferation and IgM production in vitro was markedly reduced.
| Original language | English |
|---|---|
| Pages (from-to) | 1825-1832 |
| Number of pages | 8 |
| Journal | Journal of Dairy Science |
| Volume | 81 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1998 |
Bibliographical note
Funding Information:This work was supported by a research grant from The Dairy Farmers of Canada and The Natural Sciences and Engineering Research Council of Canada (NSERC) and in part by funds from Mt. Saint Vincent University. The technical assistance of Hana James and Laura Kenny is gratefully acknowledged. The Immunocal was a generous gift from G. Bounous, McGill University (Montreal, QC, Canada).
ASJC Scopus Subject Areas
- Food Science
- Animal Science and Zoology
- Genetics
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't