TY - JOUR
T1 - Induction of protein kinase C substrates, Myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein (MRP), by amyloid β-protein in mouse BV-2 microglial cells
AU - Murphy, Anne
AU - Sunohara, Janet R.
AU - Sundaram, Meenakshi
AU - Ridgway, Neale D.
AU - McMaster, Christopher R.
AU - Cook, Harold W.
AU - Byers, David M.
PY - 2003/8/14
Y1 - 2003/8/14
N2 - Microglial activation by amyloid β-protein in senile plaques contributes to neurodegeneration in Alzheimer disease. In BV-2 microglial cells, amyloid β-protein 1-40 (Aβ 1-40) elicited a dose-dependent increase (3-4 fold) of Myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein (MRP), two protein kinase C substrates implicated in membrane-cytoskeletal alterations underlying microglial adhesion, migration, secretion, and phagocytosis. Neither MARCKS nor MRP was induced by the amyloid fragment Aβ 25-35, although both Aβ 1-40 and Aβ 25-35 caused extensive aggregation of BV-2 cells. Interferon-γ synergistically enhanced the induction by Aβ 1-40 of inducible nitric oxide synthase, but not MARCKS or MRP. Our results suggest that MARCKS and MRP may play important roles in microglia activated by amyloid peptides.
AB - Microglial activation by amyloid β-protein in senile plaques contributes to neurodegeneration in Alzheimer disease. In BV-2 microglial cells, amyloid β-protein 1-40 (Aβ 1-40) elicited a dose-dependent increase (3-4 fold) of Myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein (MRP), two protein kinase C substrates implicated in membrane-cytoskeletal alterations underlying microglial adhesion, migration, secretion, and phagocytosis. Neither MARCKS nor MRP was induced by the amyloid fragment Aβ 25-35, although both Aβ 1-40 and Aβ 25-35 caused extensive aggregation of BV-2 cells. Interferon-γ synergistically enhanced the induction by Aβ 1-40 of inducible nitric oxide synthase, but not MARCKS or MRP. Our results suggest that MARCKS and MRP may play important roles in microglia activated by amyloid peptides.
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U2 - 10.1016/S0304-3940(03)00648-7
DO - 10.1016/S0304-3940(03)00648-7
M3 - Article
C2 - 12865129
AN - SCOPUS:0038303552
SN - 0304-3940
VL - 347
SP - 9
EP - 12
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -