Isolation and characterization of 23 microsatellite loci in the stingless bee Melipona subnitida using next-generation sequencing

Isis G.B. Souza; Ian Paterson; Meghan C. McBride; Bruno A. Souza; Fabia M. Pereira; Maria T.R. Lopes; Paul Bentzen; Fabio M. Diniz

doi:10.1007/s12686-014-0347-9

Isolation and characterization of 23 microsatellite loci in the stingless bee Melipona subnitida using next-generation sequencing

Isis G.B. Souza, Ian Paterson, Meghan C. McBride, Bruno A. Souza, Fabia M. Pereira, Maria T.R. Lopes, Paul Bentzen, Fabio M. Diniz

Medicine, Science

Research output: Contribution to journal › Letter › peer-review

3 Citations (Scopus)

Abstract

We described the isolation and characterization of 23 microsatellite loci from the stingless bee (Melipona subnitida). Out of 52 microsatellite primer pairs screened, 17 loci displayed polymorphism and 6 were monomorphic. The analysis of variability was performed in 56 individuals. The number of alleles per locus ranged from 2 to 22 among populations; values for expected and observed heterozygosities ranged from 0.125 to 1.000 and from 0.121 to 0.923, respectively. These are the first microsatellite markers characterized for M. subnitida and they will be useful in obtaining estimates of population-level genetic diversity studies in a near future.

Original language	English
Pages (from-to)	239-241
Number of pages	3
Journal	Conservation Genetics Resources
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/s12686-014-0347-9
Publication status	Published - Mar 2014

Bibliographical note

Funding Information:
Sequencing was conducted on a MiSeq DNA sequencer purchased with a bequest from Elizabeth Ann Nielsen to the Marine Gene Probe Laboratory. The authors acknowledge financial support from Embrapa Macroprograma2 grant (02.11.01.029.00.00) and the Brazilian Federal Government (Science without Borders program) scholarship.

Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.

ASJC Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics

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title = "Isolation and characterization of 23 microsatellite loci in the stingless bee Melipona subnitida using next-generation sequencing",

abstract = "We described the isolation and characterization of 23 microsatellite loci from the stingless bee (Melipona subnitida). Out of 52 microsatellite primer pairs screened, 17 loci displayed polymorphism and 6 were monomorphic. The analysis of variability was performed in 56 individuals. The number of alleles per locus ranged from 2 to 22 among populations; values for expected and observed heterozygosities ranged from 0.125 to 1.000 and from 0.121 to 0.923, respectively. These are the first microsatellite markers characterized for M. subnitida and they will be useful in obtaining estimates of population-level genetic diversity studies in a near future.",

author = "Souza, {Isis G.B.} and Ian Paterson and McBride, {Meghan C.} and Souza, {Bruno A.} and Pereira, {Fabia M.} and Lopes, {Maria T.R.} and Paul Bentzen and Diniz, {Fabio M.}",

note = "Funding Information: Sequencing was conducted on a MiSeq DNA sequencer purchased with a bequest from Elizabeth Ann Nielsen to the Marine Gene Probe Laboratory. The authors acknowledge financial support from Embrapa Macroprograma2 grant (02.11.01.029.00.00) and the Brazilian Federal Government (Science without Borders program) scholarship. Publisher Copyright: {\textcopyright} 2014, Springer Science+Business Media Dordrecht.",

year = "2014",

month = mar,

doi = "10.1007/s12686-014-0347-9",

language = "English",

volume = "7",

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journal = "Conservation Genetics Resources",

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AU - Souza, Isis G.B.

AU - Paterson, Ian

AU - McBride, Meghan C.

AU - Souza, Bruno A.

AU - Pereira, Fabia M.

AU - Lopes, Maria T.R.

AU - Bentzen, Paul

AU - Diniz, Fabio M.

N1 - Funding Information: Sequencing was conducted on a MiSeq DNA sequencer purchased with a bequest from Elizabeth Ann Nielsen to the Marine Gene Probe Laboratory. The authors acknowledge financial support from Embrapa Macroprograma2 grant (02.11.01.029.00.00) and the Brazilian Federal Government (Science without Borders program) scholarship. Publisher Copyright: © 2014, Springer Science+Business Media Dordrecht.

PY - 2014/3

Y1 - 2014/3

N2 - We described the isolation and characterization of 23 microsatellite loci from the stingless bee (Melipona subnitida). Out of 52 microsatellite primer pairs screened, 17 loci displayed polymorphism and 6 were monomorphic. The analysis of variability was performed in 56 individuals. The number of alleles per locus ranged from 2 to 22 among populations; values for expected and observed heterozygosities ranged from 0.125 to 1.000 and from 0.121 to 0.923, respectively. These are the first microsatellite markers characterized for M. subnitida and they will be useful in obtaining estimates of population-level genetic diversity studies in a near future.

AB - We described the isolation and characterization of 23 microsatellite loci from the stingless bee (Melipona subnitida). Out of 52 microsatellite primer pairs screened, 17 loci displayed polymorphism and 6 were monomorphic. The analysis of variability was performed in 56 individuals. The number of alleles per locus ranged from 2 to 22 among populations; values for expected and observed heterozygosities ranged from 0.125 to 1.000 and from 0.121 to 0.923, respectively. These are the first microsatellite markers characterized for M. subnitida and they will be useful in obtaining estimates of population-level genetic diversity studies in a near future.

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Isolation and characterization of 23 microsatellite loci in the stingless bee Melipona subnitida using next-generation sequencing

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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