Isolation of cancer stem cells from cultured breast cancer cells and xenografted breast tumors based on aldehyde dehydrogenase activity

Wasundara Fernando; Derek Rowter; Raj Pranap Arun; Jaganathan Venkatesh; Paola Marcato

doi:10.1016/bs.mcb.2022.04.006

Isolation of cancer stem cells from cultured breast cancer cells and xenografted breast tumors based on aldehyde dehydrogenase activity

Wasundara Fernando, Derek Rowter, Raj Pranap Arun, Jaganathan Venkatesh, Paola Marcato

Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The heterogeneity of breast tumors is a major factor in the development, progression, and therapeutic response of breast cancer. In terms of therapy resistance, a subset of tumor cells commonly referred to as cancer stem cells (CSCs) or tumor initiating cells (TICs) have a prominent role. These cells have inherent increased tumorigenicity, self-renewal and differentiation capacity, and mechanisms for chemotherapy and radiation resistance. The importance of CSCs/TICs in cancer makes isolating and studying these cells via reliable methods critical. CSCs/TICs can be enriched for by discrete markers. Increased aldehyde dehydrogenase (ALDH) activity as detected by the Aldefluor^TM assay is a commonly used method. In this chapter, we describe the detailed methods for identification and isolation of putative CSCs/TICs from cultured cells and xenografted breast tumors using the Aldefluor^TM assay and describe the importance of the ALDH isoforms in breast cancer.

Original language	English
Title of host publication	Methods in Cell Biology
Publisher	Academic Press Inc.
DOIs	https://doi.org/10.1016/bs.mcb.2022.04.006
Publication status	Accepted/In press - 2022

Publication series

Name	Methods in Cell Biology
ISSN (Print)	0091-679X

Bibliographical note

Funding Information:
WF, RP and JV are supported by a Project Grant to PM from the Canadian Institutes of Health Research (CIHR, PJT 162313). WF is supported by a Dr. David H. Hubel Postdoctoral Fellowship through the Dalhousie Medical Research Foundation (DMRF-I3V). RPA is funded by Beatrice Hunter Cancer Research Institute Cancer Research Traineeship Program PDF fellowship. JV is supported by a PhD Fellowship for Breast Cancer Research provided through the DMRF. PM is a Senior Scientist of the Beatrice Hunter Cancer Research Institute (BHCRI). Figures were created with BioRender.com . The PDX-AIM Core of Baylor College of Medicine that supplied the PDX used in this study is supported by institutional funding from both the Advanced Technology Cores and the Dan L. Duncan Cancer Center (P30 Cancer Center Support Grant NCI-CA125123). It is also supported by a Core Facility grant from the Cancer Prevention and Research Institute of Texas (CPRIT Core Facilities Support Grant RP170691).

Publisher Copyright:
© 2022 Elsevier Inc.

ASJC Scopus Subject Areas

Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/bs.mcb.2022.04.006

Cite this

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title = "Isolation of cancer stem cells from cultured breast cancer cells and xenografted breast tumors based on aldehyde dehydrogenase activity",

abstract = "The heterogeneity of breast tumors is a major factor in the development, progression, and therapeutic response of breast cancer. In terms of therapy resistance, a subset of tumor cells commonly referred to as cancer stem cells (CSCs) or tumor initiating cells (TICs) have a prominent role. These cells have inherent increased tumorigenicity, self-renewal and differentiation capacity, and mechanisms for chemotherapy and radiation resistance. The importance of CSCs/TICs in cancer makes isolating and studying these cells via reliable methods critical. CSCs/TICs can be enriched for by discrete markers. Increased aldehyde dehydrogenase (ALDH) activity as detected by the AldefluorTM assay is a commonly used method. In this chapter, we describe the detailed methods for identification and isolation of putative CSCs/TICs from cultured cells and xenografted breast tumors using the AldefluorTM assay and describe the importance of the ALDH isoforms in breast cancer.",

author = "Wasundara Fernando and Derek Rowter and Arun, {Raj Pranap} and Jaganathan Venkatesh and Paola Marcato",

note = "Funding Information: WF, RP and JV are supported by a Project Grant to PM from the Canadian Institutes of Health Research (CIHR, PJT 162313). WF is supported by a Dr. David H. Hubel Postdoctoral Fellowship through the Dalhousie Medical Research Foundation (DMRF-I3V). RPA is funded by Beatrice Hunter Cancer Research Institute Cancer Research Traineeship Program PDF fellowship. JV is supported by a PhD Fellowship for Breast Cancer Research provided through the DMRF. PM is a Senior Scientist of the Beatrice Hunter Cancer Research Institute (BHCRI). Figures were created with BioRender.com . The PDX-AIM Core of Baylor College of Medicine that supplied the PDX used in this study is supported by institutional funding from both the Advanced Technology Cores and the Dan L. Duncan Cancer Center (P30 Cancer Center Support Grant NCI-CA125123). It is also supported by a Core Facility grant from the Cancer Prevention and Research Institute of Texas (CPRIT Core Facilities Support Grant RP170691). Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",

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N1 - Funding Information: WF, RP and JV are supported by a Project Grant to PM from the Canadian Institutes of Health Research (CIHR, PJT 162313). WF is supported by a Dr. David H. Hubel Postdoctoral Fellowship through the Dalhousie Medical Research Foundation (DMRF-I3V). RPA is funded by Beatrice Hunter Cancer Research Institute Cancer Research Traineeship Program PDF fellowship. JV is supported by a PhD Fellowship for Breast Cancer Research provided through the DMRF. PM is a Senior Scientist of the Beatrice Hunter Cancer Research Institute (BHCRI). Figures were created with BioRender.com . The PDX-AIM Core of Baylor College of Medicine that supplied the PDX used in this study is supported by institutional funding from both the Advanced Technology Cores and the Dan L. Duncan Cancer Center (P30 Cancer Center Support Grant NCI-CA125123). It is also supported by a Core Facility grant from the Cancer Prevention and Research Institute of Texas (CPRIT Core Facilities Support Grant RP170691). Publisher Copyright: © 2022 Elsevier Inc.

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N2 - The heterogeneity of breast tumors is a major factor in the development, progression, and therapeutic response of breast cancer. In terms of therapy resistance, a subset of tumor cells commonly referred to as cancer stem cells (CSCs) or tumor initiating cells (TICs) have a prominent role. These cells have inherent increased tumorigenicity, self-renewal and differentiation capacity, and mechanisms for chemotherapy and radiation resistance. The importance of CSCs/TICs in cancer makes isolating and studying these cells via reliable methods critical. CSCs/TICs can be enriched for by discrete markers. Increased aldehyde dehydrogenase (ALDH) activity as detected by the AldefluorTM assay is a commonly used method. In this chapter, we describe the detailed methods for identification and isolation of putative CSCs/TICs from cultured cells and xenografted breast tumors using the AldefluorTM assay and describe the importance of the ALDH isoforms in breast cancer.

AB - The heterogeneity of breast tumors is a major factor in the development, progression, and therapeutic response of breast cancer. In terms of therapy resistance, a subset of tumor cells commonly referred to as cancer stem cells (CSCs) or tumor initiating cells (TICs) have a prominent role. These cells have inherent increased tumorigenicity, self-renewal and differentiation capacity, and mechanisms for chemotherapy and radiation resistance. The importance of CSCs/TICs in cancer makes isolating and studying these cells via reliable methods critical. CSCs/TICs can be enriched for by discrete markers. Increased aldehyde dehydrogenase (ALDH) activity as detected by the AldefluorTM assay is a commonly used method. In this chapter, we describe the detailed methods for identification and isolation of putative CSCs/TICs from cultured cells and xenografted breast tumors using the AldefluorTM assay and describe the importance of the ALDH isoforms in breast cancer.

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Isolation of cancer stem cells from cultured breast cancer cells and xenografted breast tumors based on aldehyde dehydrogenase activity

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

UN SDGs

Access to Document

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Cite this