Knockdown of the small heat-shock protein p26 by RNA interference modifies the diapause proteome of Artemia franciscana

Hajer Salem Malitan, Alejandro M. Cohen, Thomas H. MacRae

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Embryos of the crustacean Artemia franciscana may arrest as gastrulae, forming cysts that enter diapause, which is a state of reduced metabolism and enhanced stress tolerance. Diapausing cysts survive physiological stresses for years due, in part, to molecular chaperones. p26, a small heat-shock protein, is an abundant diapause-specific molecular chaperone in cysts, and it affects embryo development and stress tolerance. p26 is therefore thought to influence many proteins in cysts, and this study was undertaken to determine how the loss of p26 by RNA interference (RNAi) affects the diapause proteome of A. franciscana. The proteome was analyzed by shot-gun proteomics coupled to differential isotopic labeling and tandem mass spectrometry. Proteins in the diapause proteome included metabolic enzymes, antioxidants, binding proteins, structural proteins, transporters, translation factors, receptors, and signal transducers. Proteins within the diapause proteome either disappeared or were reduced in amount when p26 was knocked down, or conversely, proteins appeared or increased in amount. Those proteins that disappeared may be p26 substrates, whereas the synthesis of those proteins that appeared or increased may be regulated by p26. This study provides the first global characterization of the diapause proteome of A. franciscana and demonstrates that the sHsp p26 influences proteome composition.

Original languageEnglish
Pages (from-to)471-479
Number of pages9
JournalBiochemistry and Cell Biology
Volume97
Issue number4
DOIs
Publication statusPublished - 2019

Bibliographical note

Funding Information:
The research was supported by the Natural Sciences and Engineering Research Council of Canada, Grant Number RGPIN/04882-2016. Dr. Peter Bossier, University of Gent, Ghent, Belgium, provided access to the Artemia genome database.

Publisher Copyright:
© 2019 Published by NRC Research Press.

ASJC Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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