Lambda Red Recombineering in Shigella flexneri

Adrian Herod, Julie Ryu, John Rohde

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Shigellosis remains a major cause of severe diarrheal disease and death throughout the world. Vaccine development against shigellosis has been hampered by an incomplete understanding of the molecular mechanisms by which Shigella spp. causes disease and difficulties in manipulating Shigella spp. genomes. While homologous recombination protocols for the construction of precise gene deletions exist, construction of mutants in S. flexneri has not become commonplace. We describe the steps for construction of gene deletions using λ-red recombination using tools that we have developed in our laboratory.

Original languageEnglish
Pages (from-to)9-21
Number of pages13
JournalMethods in Molecular Biology
Volume2523
DOIs
Publication statusPublished - 2022

Bibliographical note

Publisher Copyright:
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

  • Molecular Biology
  • Genetics

PubMed: MeSH publication types

  • Journal Article

Fingerprint

Dive into the research topics of 'Lambda Red Recombineering in Shigella flexneri'. Together they form a unique fingerprint.

Cite this