Abstract
Shigellosis remains a major cause of severe diarrheal disease and death throughout the world. Vaccine development against shigellosis has been hampered by an incomplete understanding of the molecular mechanisms by which Shigella spp. causes disease and difficulties in manipulating Shigella spp. genomes. While homologous recombination protocols for the construction of precise gene deletions exist, construction of mutants in S. flexneri has not become commonplace. We describe the steps for construction of gene deletions using λ-red recombination using tools that we have developed in our laboratory.
Original language | English |
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Pages (from-to) | 9-21 |
Number of pages | 13 |
Journal | Methods in Molecular Biology |
Volume | 2523 |
DOIs | |
Publication status | Published - 2022 |
Bibliographical note
Publisher Copyright:© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
ASJC Scopus Subject Areas
- Molecular Biology
- Genetics
PubMed: MeSH publication types
- Journal Article