Large-scale discovery of previously undetected microRNAs specific to human liver

Brenda C. Minatel; Victor D. Martinez; Kevin W. Ng; Adam P. Sage; Tomas Tokar; Erin A. Marshall; Christine Anderson; Katey S.S. Enfield; Greg L. Stewart; Patricia P. Reis; Igor Jurisica; Wan L. Lam

doi:10.1186/s40246-018-0148-4

Large-scale discovery of previously undetected microRNAs specific to human liver

Brenda C. Minatel, Victor D. Martinez, Kevin W. Ng, Adam P. Sage, Tomas Tokar, Erin A. Marshall, Christine Anderson, Katey S.S. Enfield, Greg L. Stewart, Patricia P. Reis, Igor Jurisica, Wan L. Lam

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are crucial regulators of gene expression in normal development and cellular homeostasis. While miRNA repositories contain thousands of unique sequences, they primarily contain molecules that are conserved across several tissues, largely excluding lineage and tissue-specific miRNAs. By analyzing small non-coding RNA sequencing data for abundance and secondary RNA structure, we discovered 103 miRNA candidates previously undescribed in liver tissue. While expression of some of these unannotated sequences is restricted to non-malignant tissue, downregulation of most of the sequences was detected in liver tumors, indicating their importance in the maintenance of liver homeostasis. Furthermore, target prediction revealed the involvement of the unannotated miRNA candidates in fatty-acid metabolism and tissue regeneration, which are key pathways in liver biology. Here, we provide a comprehensive analysis of the undiscovered liver miRNA transcriptome, providing new resources for a deeper exploration of organ-specific biology and disease.

Original language	English
Article number	16
Journal	Human Genomics
Volume	12
Issue number	1
DOIs	https://doi.org/10.1186/s40246-018-0148-4
Publication status	Published - Jan 26 2018
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by grants from the Canadian Institutes for Health Research (CIHR FRN-143345). BCM, VDM, APS, EAM, and CA are supported by scholarships from the University of British Columbia. EAM and APS are also supported by scholarships from CIHR. IJ is in part supported by Canada Research Chair Program (#225404), Ontario Research Fund (GL2-01-030) and Canada Foundation for Innovation (CFI #225404, #30865).

Publisher Copyright:
© 2018 The Author(s).

ASJC Scopus Subject Areas

Molecular Medicine
Molecular Biology
Genetics
Drug Discovery

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abstract = "MicroRNAs (miRNAs) are crucial regulators of gene expression in normal development and cellular homeostasis. While miRNA repositories contain thousands of unique sequences, they primarily contain molecules that are conserved across several tissues, largely excluding lineage and tissue-specific miRNAs. By analyzing small non-coding RNA sequencing data for abundance and secondary RNA structure, we discovered 103 miRNA candidates previously undescribed in liver tissue. While expression of some of these unannotated sequences is restricted to non-malignant tissue, downregulation of most of the sequences was detected in liver tumors, indicating their importance in the maintenance of liver homeostasis. Furthermore, target prediction revealed the involvement of the unannotated miRNA candidates in fatty-acid metabolism and tissue regeneration, which are key pathways in liver biology. Here, we provide a comprehensive analysis of the undiscovered liver miRNA transcriptome, providing new resources for a deeper exploration of organ-specific biology and disease.",

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note = "Funding Information: This work was supported by grants from the Canadian Institutes for Health Research (CIHR FRN-143345). BCM, VDM, APS, EAM, and CA are supported by scholarships from the University of British Columbia. EAM and APS are also supported by scholarships from CIHR. IJ is in part supported by Canada Research Chair Program (#225404), Ontario Research Fund (GL2-01-030) and Canada Foundation for Innovation (CFI #225404, #30865). Publisher Copyright: {\textcopyright} 2018 The Author(s).",

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AU - Marshall, Erin A.

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AU - Jurisica, Igor

AU - Lam, Wan L.

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N2 - MicroRNAs (miRNAs) are crucial regulators of gene expression in normal development and cellular homeostasis. While miRNA repositories contain thousands of unique sequences, they primarily contain molecules that are conserved across several tissues, largely excluding lineage and tissue-specific miRNAs. By analyzing small non-coding RNA sequencing data for abundance and secondary RNA structure, we discovered 103 miRNA candidates previously undescribed in liver tissue. While expression of some of these unannotated sequences is restricted to non-malignant tissue, downregulation of most of the sequences was detected in liver tumors, indicating their importance in the maintenance of liver homeostasis. Furthermore, target prediction revealed the involvement of the unannotated miRNA candidates in fatty-acid metabolism and tissue regeneration, which are key pathways in liver biology. Here, we provide a comprehensive analysis of the undiscovered liver miRNA transcriptome, providing new resources for a deeper exploration of organ-specific biology and disease.

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Large-scale discovery of previously undetected microRNAs specific to human liver

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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