Lepeophtheirus salmonis: Characterization of prostaglandin E2 in secretory products of the salmon louse by RP-HPLC and mass spectrometry

M. D. Fast; N. W. Ross; C. A. Craft; S. J. Locke; S. L. MacKinnon; S. C. Johnson

doi:10.1016/j.exppara.2004.04.001

Lepeophtheirus salmonis: Characterization of prostaglandin E₂ in secretory products of the salmon louse by RP-HPLC and mass spectrometry

M. D. Fast, N. W. Ross, C. A. Craft, S. J. Locke, S. L. MacKinnon, S. C. Johnson

Medicine

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

Lepeophtheirus salmonis is an ectoparasitic copepod that causes serious disease outbreaks in both wild and farmed salmonids. As the relationship between L. salmonis and its hosts is not well understood, the current investigation was undertaken to investigate whether any immunomodulatory compounds could be identified from secretions of L. salmonis. By incubating live L. salmonis adults with the neurotransmitter dopamine in seawater, we were able to obtain secretions from the parasite. These were analyzed by RP-HPLC column, as well as LC-MS. L. salmonis secretions contained a compound with the same retention time and mass of PGE₂. The identity of this compound as PGE₂ was confirmed by MS - in source dissociation. The concentrations of PGE ₂ in L. salmonis secretions ranged from 0.2 to 12.3ng/individual and varied with incubation temperature and time kept off the host. Prostaglandin E₂ is a potent vasodilator and thought to aid in parasite evasion from host immune responses. This is the first reported evidence of prostaglandin production in parasitic copepod secretions and its implications for the host-parasite relationship are discussed. Index Descriptors and Abbreviations: prostaglandin (PG), sea lice, copepod, Lepeophtheirus salmonis, immunomodulation, secretory/excretory product (SEP), secretory products (SPs), dopamine, liquid chromatography-mass spectrometry (LC-MS), reverse phased-high performance liquid chromatography (RP-HPLC)

Original language	English
Pages (from-to)	5-13
Number of pages	9
Journal	Experimental Parasitology
Volume	107
Issue number	1-2
DOIs	https://doi.org/10.1016/j.exppara.2004.04.001
Publication status	Published - May 2004

Bibliographical note

Funding Information:
The authors thank Dr. John Burka, Jennifer Ramsey, and other UPEI-AVC staff, Heritage Salmon Ltd., Stolt Sea Farms, Holly Beaman, Dr. Fred Whoriskey, and Jonathan Carr of the Atlantic Salmon Federation for all their assistance in obtaining parasite samples. This work was funded as part of an Industrial Post Graduate Scholarship from NSERC and the Microtek International collaboration with the Institute for Marine Biosciences. The manuscript is NRCC Publication #42390.

ASJC Scopus Subject Areas

Parasitology
Immunology
Infectious Diseases

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.exppara.2004.04.001

Cite this

@article{f009350a6fe446e2aa47f45547e37da6,

title = "Lepeophtheirus salmonis: Characterization of prostaglandin E2 in secretory products of the salmon louse by RP-HPLC and mass spectrometry",

abstract = "Lepeophtheirus salmonis is an ectoparasitic copepod that causes serious disease outbreaks in both wild and farmed salmonids. As the relationship between L. salmonis and its hosts is not well understood, the current investigation was undertaken to investigate whether any immunomodulatory compounds could be identified from secretions of L. salmonis. By incubating live L. salmonis adults with the neurotransmitter dopamine in seawater, we were able to obtain secretions from the parasite. These were analyzed by RP-HPLC column, as well as LC-MS. L. salmonis secretions contained a compound with the same retention time and mass of PGE2. The identity of this compound as PGE2 was confirmed by MS - in source dissociation. The concentrations of PGE 2 in L. salmonis secretions ranged from 0.2 to 12.3ng/individual and varied with incubation temperature and time kept off the host. Prostaglandin E2 is a potent vasodilator and thought to aid in parasite evasion from host immune responses. This is the first reported evidence of prostaglandin production in parasitic copepod secretions and its implications for the host-parasite relationship are discussed. Index Descriptors and Abbreviations: prostaglandin (PG), sea lice, copepod, Lepeophtheirus salmonis, immunomodulation, secretory/excretory product (SEP), secretory products (SPs), dopamine, liquid chromatography-mass spectrometry (LC-MS), reverse phased-high performance liquid chromatography (RP-HPLC)",

author = "Fast, {M. D.} and Ross, {N. W.} and Craft, {C. A.} and Locke, {S. J.} and MacKinnon, {S. L.} and Johnson, {S. C.}",

note = "Funding Information: The authors thank Dr. John Burka, Jennifer Ramsey, and other UPEI-AVC staff, Heritage Salmon Ltd., Stolt Sea Farms, Holly Beaman, Dr. Fred Whoriskey, and Jonathan Carr of the Atlantic Salmon Federation for all their assistance in obtaining parasite samples. This work was funded as part of an Industrial Post Graduate Scholarship from NSERC and the Microtek International collaboration with the Institute for Marine Biosciences. The manuscript is NRCC Publication #42390. ",

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T2 - Characterization of prostaglandin E2 in secretory products of the salmon louse by RP-HPLC and mass spectrometry

AU - Fast, M. D.

AU - Ross, N. W.

AU - Craft, C. A.

AU - Locke, S. J.

AU - MacKinnon, S. L.

AU - Johnson, S. C.

N1 - Funding Information: The authors thank Dr. John Burka, Jennifer Ramsey, and other UPEI-AVC staff, Heritage Salmon Ltd., Stolt Sea Farms, Holly Beaman, Dr. Fred Whoriskey, and Jonathan Carr of the Atlantic Salmon Federation for all their assistance in obtaining parasite samples. This work was funded as part of an Industrial Post Graduate Scholarship from NSERC and the Microtek International collaboration with the Institute for Marine Biosciences. The manuscript is NRCC Publication #42390.

PY - 2004/5

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N2 - Lepeophtheirus salmonis is an ectoparasitic copepod that causes serious disease outbreaks in both wild and farmed salmonids. As the relationship between L. salmonis and its hosts is not well understood, the current investigation was undertaken to investigate whether any immunomodulatory compounds could be identified from secretions of L. salmonis. By incubating live L. salmonis adults with the neurotransmitter dopamine in seawater, we were able to obtain secretions from the parasite. These were analyzed by RP-HPLC column, as well as LC-MS. L. salmonis secretions contained a compound with the same retention time and mass of PGE2. The identity of this compound as PGE2 was confirmed by MS - in source dissociation. The concentrations of PGE 2 in L. salmonis secretions ranged from 0.2 to 12.3ng/individual and varied with incubation temperature and time kept off the host. Prostaglandin E2 is a potent vasodilator and thought to aid in parasite evasion from host immune responses. This is the first reported evidence of prostaglandin production in parasitic copepod secretions and its implications for the host-parasite relationship are discussed. Index Descriptors and Abbreviations: prostaglandin (PG), sea lice, copepod, Lepeophtheirus salmonis, immunomodulation, secretory/excretory product (SEP), secretory products (SPs), dopamine, liquid chromatography-mass spectrometry (LC-MS), reverse phased-high performance liquid chromatography (RP-HPLC)

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