Localization of neutral, magnesium-stimulated sphingomyelinase in plasma membrane of cultured neuroblastoma cells

Matthew W. Spence; Jan Wakkary; Joe T.R. Clarke; Harold W. Cook

doi:10.1016/0304-4165(82)90321-X

Localization of neutral, magnesium-stimulated sphingomyelinase in plasma membrane of cultured neuroblastoma cells

Matthew W. Spence, Jan Wakkary, Joe T.R. Clarke, Harold W. Cook

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

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Abstract

A parallel is shown between the distribution of neutral sphingomyelinase and plasma membrane enzymes (5′-nucleotidase and (Na⁺ + K⁺)-activated ATPase) in cultured neuroblastoma cells. In contrast there is no evidence of localization in lysosomes (β-hexosaminidase and acid sphingomyelinase), mitochondria (carnitine palmitoyltransferase), or cytosol. Activity in the microsomal fraction is attributed primarily to plasma membrane contamination.

Original language	English
Pages (from-to)	162-164
Number of pages	3
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	719
Issue number	1
DOIs	https://doi.org/10.1016/0304-4165(82)90321-X
Publication status	Published - Oct 28 1982

Bibliographical note

Funding Information:
The authors acknowledge the expert technical assistance of Mr. Alfred Archibald and Mr. Robert Zwicker. This research was supported in part by a grant-in-aid from the Medical Research Council of Canada (PG-16). H.W.C. is a Scholar and M.W.S. is a Career Investigator of the Medical Research Council of Canada.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0304-4165(82)90321-X

Cite this

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abstract = "A parallel is shown between the distribution of neutral sphingomyelinase and plasma membrane enzymes (5′-nucleotidase and (Na+ + K+)-activated ATPase) in cultured neuroblastoma cells. In contrast there is no evidence of localization in lysosomes (β-hexosaminidase and acid sphingomyelinase), mitochondria (carnitine palmitoyltransferase), or cytosol. Activity in the microsomal fraction is attributed primarily to plasma membrane contamination.",

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AU - Spence, Matthew W.

AU - Wakkary, Jan

AU - Clarke, Joe T.R.

AU - Cook, Harold W.

N1 - Funding Information: The authors acknowledge the expert technical assistance of Mr. Alfred Archibald and Mr. Robert Zwicker. This research was supported in part by a grant-in-aid from the Medical Research Council of Canada (PG-16). H.W.C. is a Scholar and M.W.S. is a Career Investigator of the Medical Research Council of Canada.

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Y1 - 1982/10/28

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Localization of neutral, magnesium-stimulated sphingomyelinase in plasma membrane of cultured neuroblastoma cells

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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